The ion microscope was used to study potassium and calcium distributions in the main motor organ of Mimosa pudica L. The cortex of the motor organ has two cell types differing in location, structure, and ion distribution. Histochemical features portrayed in ion micrographs were plainly correlated with structures seen in the light and electron microscopes.
A secondary ion mass image processing system (SIMIPS) is presented as a quantitative image analysis tool, with emphasis on an efficient man-machine interface. The combined applications of digital image processing and pattern recognition ensure an intelligent problem-resolving scheme and optimal extraction of information. The system performance is evaluated,… (More)
Both elemental distribution and ion transport in cultured cells have been imaged by ion microscopy. Morphological and chemical information was obtained with a spatial resolution of approximately 0.5 micron for sodium, potassium, calcium, and magnesium in freeze-fixed, cryofractured, and freeze-dried normal rat kidney cells and Chinese hamster ovary cells.… (More)
An ion microscope has been used to demonstrate that the calcium ion is present in the amyloplasts of root-cap cells of corn, pea, and lettuce. The localization of calcium in the gravity-sensing organelle suggests a possible role of calcium in the gravity-sensing mechanism of plant roots.
Clinical studies of the treatment of glioma and cutaneous melanoma using boron neutron capture therapy (BNCT) are currently taking place in the USA, Europe and Japan. New BNCT clinical facilities are under construction in Finland, Sweden, England and California. The observation of transient acute effects in the oral mucosa of a number of glioma patients… (More)
Calcium to calcium exchange between intracellular and extracellular pools has been imaged directly in individual cells using stable 44Ca (98.78% enrichment) in the nutrient medium and an isotopic imaging technique, ion microscopy. Observations were made by imaging mass 40 to determine the native intracellular calcium (40Ca), and mass 44 to localize the 44Ca… (More)