Georg Lambertsen

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A method for the separation from fish tissues of the four main glycerophospholipid classes, phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol and phosphatidylserine, using adsorption high-performance liquid chromatography with ultraviolet detection and consecutive gas chromatographic analysis, based on one injection for their fatty acid(More)
The liver metabolism of erucic acid, 22:1 n-9, was studied in rat livers perfused with 14-14C-labelled erucic acid for 15 and 60 min, determining the distribution of radioactivity in the perfusate and in the lipids of the liver cell organelles. The rats were adapted to a high-fat diet containing either partially hydrogenated marine oil (PHMO) or palm oil(More)
Rats were fed for 8 weeks high fat diets containing 20% of either palm oil (PO) or linoleic-supplemented partially hydrogenated marine oil (PHMO). Hepatocytes were isolated from rats fasted overnight. Incubations were performed with albumin bound [10-14C] oleic acid and [14-14C] erucic acid, respectively, for 15 or 60 min. The cell viability was better than(More)
A chemical method for the analysis of naturally occurring vitamin D is proposed. The unsaponifiable matter of oils and tissues is prepared, cholesterol is partly removed by double precipitation at low temperature in methanol. The vitamin D fraction is collected on an adsorption column by high performance liquid chromatography. The fraction is further(More)
Female pigs, fed diets in which 42% of the caloric intake came from either rapeseed oil, fish oil, partially hydrogenated fish oil, partially hydrogenated soybean oil or lard, were killed after one week, five weeks, six months and one year. Type of fat or length of feeding did not affect the cardiac content of total fat which was normal in all animals. The(More)
Cod (mean start weight of 26 g) were fed three diets for 15 months, each based on a dry pellet coated at a level of 9g/100 g with soybean oil, capelin oil or sardine oil. The fatty acid compositions of neutral lipids and four glycerophospholipids of white muscle, liver, gills and heart were determined. The fatty acid composition of dietary lipids influenced(More)
1. Ubiquinone contents were determined in species of marine invertebrates, and in heart, red and white muscle and liver of three species of fish. 2. Three different methods of determination were compared, based on spectrophotometry, reduction and a reaction with the dimethoxy groups of ubiquinone. 3. Using ubiquinone homologues 6-10 prepared from beef heart(More)