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OBJECTIVE To investigate whether human preadipocytes possess a complete functional renin angiotensin system. MEASUREMENTS Gene expression of angiotensinogen, renin, renin binding protein, angiotensin converting enzyme (ACE) and angiotensin II (ang II) receptor type 1 in human preadipocytes; ACE protein and ang II production of human adipose tissue stromal(More)
3T3-L1-adipocytes produce the adipocyte complement related protein of 30 kD (Acrp30), which is also designated as AdipoQ. In order to study the expression and secretion of the human homologue of this protein, apM1 (adipose Most abundant gene transcript 1, also named gelatin-binding protein of 28 kD [GBP28] or adiponectin), a polyclonal antibody was(More)
The metabolic functionality of adipose tissue is intimately dependent on local communication between various cell types. It influences not only the equilibrium between lipogenesis and lipolysis but also between hypertrophic and hyperplastic growth, thereby determining the role adipose tissue plays in the insulin resistance syndrome.
This paper presents a rigorous derivation of a theory for the calculation of the frequency-dependent dielectric properties of each component of the system protein/water/ions with the aim of enabling comparison to experimentally determined dielectric properties. We apply this theory to a very long (13.1 ns) molecular dynamics simulation of an HIV1 zinc(More)
Fetuin is frequently in use as a serum substitute especially during the culture of differentiating preadipocyte cell lines. We show here, that the commercially available crude Pedersen fetuin contains a factor permissive for adipose conversion. It can be separated from fetuin by dialysis at acid pH. When 3T3-L1 cells are cultured in the presence of purified(More)
During their development from progenitor cells, adipocytes not only express enzymatic activities necessary for the storage of triglycerides, but also achieve the capability to produce a number of endocrine factors such as leptin, tumor necrosis factor alpha (TNFalpha), complement factors, adiponectin/adipoQ, plasminogen activator inhibitor-1 (PAI-1),(More)
The adipose conversion of 3T3-L1 fibroblasts to fully developed adipocytes depends on the presence of serum in the culture medium. This study demonstrates that human serum or plasma also effectively promote the adipose conversion of 3T3-L1 cells. At rising serum concentrations this effect is abolished however by the presence of antiadipogenic factors. Sera(More)
The adipose conversion of 3T3-Li cells depends on a serum factor present in high amounts in fetal calf serum, which is heat stable and can be extracted from serum by ethanol precipitation. Sera of two genetically obese rodent species, fa/fa Zucker rats and C57Bl/KsJ-db/db mice, contain a high adipogenic activity which is very similar to that found in fetal(More)
Adipose conversion of 3T3-L1 cells depends on adipogenic factors present in serum. In order to find their origin, adipogenic activity in extracts from adrenals, kidneys, testes, ovaries, liver, spleen, sceletal muscle and adipose tissue of the rat was studied. If tissues were homogenized with aqueous buffers at low pH, no adipogenic activity could be(More)
Shifts in the distribution of the monovalent cations Na+ and K+ between the extra-and intracellular space seem to be important for the secretory response of the β-cell. An attempt was therefore made to study the enzyme responsible for monovalent cation transport, the (NaK)-activated ATPase. In the presence of NaN3 as inhibitor of the mitochondrial(More)