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In this study, we evaluated the permeation of piperacillin (PIPC), imipenem (IPM), amikacin (AKM), gentamicin (GM), ofloxacin (OFLX), levofloxacin (LVFX), ciprofloxacin (CPFX) and sparfloxacin (SPFX) through Pseudomonas aeruginosa biofilm with a simple new method. Bacteria used were a leucine-requiring mucoid mutant. Bacteria were grown on the membrane of a(More)
The growth rate of biofilm bacteria of a leucine-requiring mutant Pseudomonas aeruginosa HU1 was regulated by the leucine concentration in a chemically-defined medium. The semiquantitative measurement of glycocalyx and scanning electron microscopy revealed that the kinetics of HU1-biofilm formation were dependent on the incubation time and the leucine(More)
Using an experimental in vitro culture system, we investigated the effect of clarithromycin on biofilm formation by a leucine-requiring Pseudomonas aeruginosa mutant strain (HU1). Biofilm formation on celldesks in a chemically defined medium was assessed by viable cell count as well as by measurement of glycocalyx production and scanning(More)
In order to elucidate the factors influencing glucose intolerance. 100 g oral glucose tolerance tests serially performed at annual physical examinations over a 10-year period were studied. On the initial examination in 356 subjects, 44.8% were normal and 7.1% diabetic according to the criteria of 100 g OGTT recommended by the Japan Diabetes Society in 1970.(More)
Fucoidan was extracted from the seaweed Ascophyllum nodosum with boiling water and purified by repeated precipitation steps. Increasing doses of fucoidan (1.2--200 mg) were injected together with 360 nmol 59Fe-(FeCl3) or 60Co-(CoCl2) into tied-off jejunal segments of two groups of rats fed either a normal (160 mg Fe/kg) or a low iron diet (5 mg/kg).(More)
Oral administration of N-[4-(3-ethoxy-2-hydropropoxy)phenyl] acrylamide (EHA) induced selective granule cell destruction in the granular layer of the cerebellar cortex together with neurological signs, such as delayed righting reflex, gait or truncal ataxia, and convulsion. Neuropathologically, it caused multifocal granule cell destruction with nuclear(More)
An explanation of increased hydrolysis of the β-(1,4)-glycosidic linkages of grafted cellulose using molecular modeling" (2006). Faculty Publications-Textiles, Merchandising and Fashion Design. Paper 34. Abstract Grafting various groups onto cellulose is found to substantially increase acid hydrolysis of the b-(1,4)-glycosidic linkages. Molecular mod-eling(More)
  • G Tanaka
  • 1992
During the 12 year period from 1978 to 1990, 112 mature newborn and premature infants were diagnosed as sepsis in our nursery. The first case of MRSA sepsis was found in 1985. Since then, cases abruptly increased in number and 31 cases were found in total. Seven cases died and 24 were cured. Antibiotics such as AMK, MINO, IPM were effective. As the(More)