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Functional analysis of bovine foamy virus accessory protein and Borf-1 responsive elements in long terminal repeat.
Regulatory elements that mediate transactivation by Tas in the internal promoter of bovine foamy virus.
Generation of Full-genome SHIV-KB9 Clone and Its Replication in Human and Monkey Peripheral Blood Mononuclear Cells
The infectivity of the full-length SHIV-KB9 in human peripheral blood mononuclear cells (PBMC), cynomolgus and rhesus monkey PBMC was characterized by polymerase chain reaction (PCR), reverse transcription PCR (RT-PCR) and Simian immunodeficiency virus p27 gag antigen titration assay.
Cloning and functional analysis of the internal promoter of a bovine foamy virus.
Bovine foamy virus is a member of spumavirus that encodes three retroviral genes, pag, pol and env for virion proteins as well as additional open reading frames, which suggests that different transactivation mechanisms were involved in regulating BFV LTR and IP.
Membrane-spanning domain of bovine foamy virus transmembrane protein having cytotoxicity
The bacterial amount and living bacteria analysis revealed that the cytotoxicity of TM was dependent on its MSD, especially on the stretches of hydrophobic amino acids, and western blotting analysis showed that TM3 protein was purified with affinity purification.
CLONING AND HIGH LEVEL EXPRESSION OF GLUCOSE DEHYDROGENASE GENE FROM BACILLUS SUBTILIS IN E. COLI
- Geng Yunqi
The results showed that GDH, a widely used enzyme in industry, has been expressed efficiently in E. coli.
The Study on the Infection of Rabbits with Bovine Foamy Virus 3026
- Yu Hong, Kong Xiao-hong, Xuan Chenghao, W. Jinzhong, Chen Qi—rain, Geng Yunqi
- 25 December 2003
Rabbits were injected with a single intravenation of Bovine foamy virus 3026-infected cells in the ear and monitored for sera-conversion serologically up to 1 year and showed sustained BFV3026-specific humoral immune response, which might be useful for studying the interaction between BFV and BIV.
Construction and Characterization of SHIV Carrying HIV-1 Capsid
Chimeric SHIV virions have complete structures, including genomic RNA, reverse transcriptase, core proteins and envelope with glycoproteins, and could Absorb and enter into MT4 cells and complete the course of reverse transcription.
Role of HBx During the Super-Infection of HBV and HIV
- Geng Yunqi
The advances in the study of the role of HBx during the super-infection of HBV and HIV are reviewed.
Expression of Matrix and Capsid Protein of Bovine Immunodeficiency Virus
The matrix and the capsid are main structural proteins of Bovine immunodeficency virus (BIV) and play important roles in virus life cycle and western blotting analysis showed that the antiserum could specifically recognize the native HIV CA protein.