Gene Scalarone

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Enzyme-linked immunosorbent assay (ELISA) comparative studies are described in which 10 Blastomyces dermatitidis antigenic preparations from human, dog, and soil strains were used for the detection of rabbit serum antibodies directed against B. dermatitidis killed yeast cells of human and dog origin as well as detection of antibodies in human serum samples.(More)
The phenomenon of persistent suppression of Candida albicans yeast cell growth after short drug exposures (postantifungal effect, PAFE) was determined by performing comparative studies using different concentrations of 5-fluorocytosine (5-FC), amphotericin B (AMPH) and miconazole (MCZ). An in vitro turbidometric method was used to measure cell growth and to(More)
Blastomyces dermatitidis (dog isolate T-58) yeast phase lysate antigen was concentrated and separated by Rotofor preparative isoelectric focusing cell (Bio-Rad). The pH values of the fractions were determined and equilibrated to pH 7.2 and then analysed by enzyme-linked immunosorbent assay using horseradish peroxidase enzyme system against serum specimens(More)
A competitive binding inhibition enzyme linked immunosorbent assay (ELISA) was used to detect Blastomyces dermatitidis antigens in urine specimens from dogs with blastomycosis. Sera from rabbits immunized with B. dermatitidis killed whole yeast cells were used as the primary antibody in the competitive ELISA. This initial study was performed to determine if(More)
A yeast phase histoplasmin, designated CYL, was studied in 156 humans living in areas of the United States endemic for histoplasmosis, coccidioidomycosis, or both fungal entities. Comparisons were made with Food and Drug Administration reference mycelial histoplasmin. Among persons without concurrent spherulin sensitivity, reactor rates and sizes were the(More)
Controlled yeast lysate (CYL) and controlled mycelial lysate (CML) histoplasmins were produced from Histoplasma capsulatum grown in a nutritionally lean, chemically defined medium. The lysates were assayed for skin-test activity in guinea pigs sensitized by infection with the homologous organism. In some studies, nonliving vaccine preparations were employed(More)
Yeast phase lysate antigens, prepared from three isolates of Blastomyces dermatitidis (T-58, Tennessee dog; 48089, Zaire human; ERC-2, Wisconsin dog) were assayed for their ability to detect antibodies in human sera, dog sera and sera from rabbits immunized with each of the lysate antigens. The dog sera were from animals diagnosed with blastomycosis from(More)
Yeast phase lysate antigens prepared from different isolates of Blastomyces dermatitidis (T-58, dog-Tennessee; T-27, polar bear-Tennessee; ERC-2, dog-Wisconsin; ER-3, woodpile-Wisconsin) were compared with respect to the detection of antibodies (indirect enzyme-linked immunosorbent assay-ELISA, peroxidase system) in 126 serial serum specimens(More)
Yeast phase lysate antigens, prepared from seven isolates of Blastomyces dermatitidis (dogs, T-58, M-98; human, Le; soil, S; sea lion, SL; polar bear, PB; cat, C) were assayed by enzyme-linked immunosorbent assay (ELISA) to detect antibodies in human sera. The sera were from individuals potentially exposed to B. dermatitidis while working on a prairie dog(More)