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The Human-Computer Interaction Laboratory (HCIL) of the University of Maryland and NASA have collaborated over three years to refine and apply user interface research concepts developed at HCIL in order to improve the usability of NASA data services. The research focused on dynamic query user interfaces, visualization, and overview + preview designs. An(More)
We describe in detail a direct assay for the substrate-inactivated DNA-repair enzyme, O6-methylguanine-DNA methyltransferase (O6-MT), which measures the transfer of radiolabelled methyl groups from a prepared O6-methylguanine-DNA substrate to the protein fraction of an enzyme-containing cell/tissue extract. This assay, a modification of a previously(More)
Eukaryotic cells contain numerous small-molecular-mass GTP-binding proteins, but the processes that they regulate are not known. Different members of this protein family appear to be associated with specific GTPase-activating proteins (GAPs), and we have previously reported the identification of a cytoplasmic GAP (rho GAP) that stimulates the GTPase(More)
The protein kinase inhibitor H7 [1-5(isoquinolinesulfonyl-2-methylpiperazine)] together with a temperature shift to 42 degrees C was found to reproducibly and efficiently induce differentiation of avian erythroblasts transformed with the avian erythroblastosis virus containing v-erbA and a temperature-sensitive v-erbB oncogene. Although a temperature shift(More)
DNA repair by O6-methylguanine-DNA methyltransferase (O6-MT) is accomplished by removal by the enzyme of the methyl group from premutagenic O6-methylguanine-DNA, thereby restoring native guanine in DNA. The methyl group is transferred to an acceptor site cysteine thiol group in the enzyme, which causes the irreversible inactivation of O6-MT. We detected a(More)
The Global Change Master Directory (GCMD) is an earth science information repository that tracks research data on global climatic change. Building a directory of Earth science metadata that allows the exchange of metadata content among partner organizations is challenging because of the complex issues involved in supporting heterogeneous metadata schema,(More)
The Global Change Master Directory (GCMD) is an earth science repository that specifically tracks research data on global climatic change. The GCMD is migrating from a centralized architecture to a globally distributed replicated heterogeneous federated system. One of the greatest challenges facing database research is the integration of heterogeneous(More)
We have raised antibodies against a synthetic dodecapeptide corresponding to the carboxyl terminus of the predicted met gene product. Phosphorylation of 60 kDa and 65 kDa proteins on tyrosine residues was observed when immunoprecipitates of cells containing the activated human met gene were incubated with [gamma-32P]ATP. Phosphoproteins with the same(More)
This demonstration focuses on the implementation of an asynchronous transaction protocol [1,6, 8] which satisfies the requirement of replicating DlF entries through the internet across continents in the International Directory Network (IDN). We have designed a Primary Site Distribution protocol which is asynchronous but active i.e. participating sites in(More)
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