Gary R. Gunther

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Chemical derivatization of tetrameric concanavalin A (Con A) with succinic anhydride or acetic anhydride converts the protein to a dimeric molecule without altering its carbohydrate-binding specificity. At low concentrations, the dose-response curves for the mitogenic stimulation of mouse spleen cells by native Con A and succinyl-Con A are similar. Above(More)
Colchicine, vinblastine, and vincristine inhibit the mitogenic stimulation of lymphocytes by concanavalin A as measured by the incorporation of [3H]thymidine and the appearance of blast cells. The inhibitory effect of colchicine could not be accounted for by diminution in cell viability or by metaphase arrest of mitosis in the stimulated cells. Moreover,(More)
To investigate the inhibitory effect of adenosine released by endothelium on neutrophil superoxide (O2-) production, we treated confluent monolayers of cultured human umbilical vein endothelial cells with the enzyme adenosine deaminase, and then added human neutrophils. Superoxide (O2-) production by human neutrophils stimulated with 10(-6) M(More)
The kinetics of cellular commitment in the stimulation of lymphocytes by concanavalin A (Con A) has been analyzed by measurement of DNA synthesis, autoradiography, and histologic staining techniques. If the competitive inhibitor alpha-methyl-D-mannoside (alphaMM) is introduced into cultures of mouse spleen cells at various times after the addition of Con A,(More)
When pancreatic acini are exposed to the peptide caerulein (an analogue of the hormone cholecystokinin) or the cholinergic agonist carbamylcholine, they exhibit a rapid release of intracellular Ca2+ and a rise in the level of cGMP, accompanied by discharge of secretory proteins. The phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) stimulates protein(More)