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The Cellulose Synthase-Like D (CslD) genes have important, although still poorly defined, roles in cell wall formation. Here, we show an unexpected involvement of CslD1 from maize (Zea mays) in cell division. Both division and expansion were altered in the narrow-organ and warty phenotypes of the csld1 mutants. Leaf width was reduced by 35%, due mainly to a(More)
To assess the cell wall's role as a barrier to intracellular delivery, wild-type Chlamydomonas reinhardtii algal cells and mutant cells lacking a cell wall were exposed to electroporation or sonication. Flow cytometry determined intracellular uptake of calcein and bovine serum albumin (BSA) and loss of cell viability as functions of electroporation(More)
  • Johan Wadenbäck, David H Clapham, Deborah Craig, Ronald Sederoff, Gary F Peter, Sara von Arnold +1 other
  • 2005
BACKGROUND The need to perform microarray experiments with small amounts of tissue has led to the development of several protocols for amplifying the target transcripts. The use of different amplification protocols could affect the comparability of microarray experiments. RESULTS Here we compare expression data from Pinus taeda cDNA microarrays using(More)
In the rapidly evolving field of genomics, many clustering and classification methods have been developed and employed to explore patterns in gene expression data. Biologists face the choice of which clustering algorithm(s) to use and how to interpret different results from the various clustering algorithms. No clear objective criteria have been developed(More)
Genetic analysis of wood chemical composition is often limited by the cost and throughput of direct analytical methods. The speed and low cost of Fourier transform near infrared (FT-NIR) overcomes many of these limitations, but it is an indirect method relying on calibration models that are typically developed and validated with small sample sets. In this(More)
Fusiform rust resistance can involve gene-for-gene interactions where resistance (Fr) genes in the host interact with corresponding avirulence genes in the pathogen, Cronartium quercuum f.sp. fusiforme (Cqf). Here, we identify trees with Fr genes in a loblolly pine population derived from a complex mating design challenged with two Cqf inocula (one gall and(More)
  • Jeffrey R. Fedenko, John E. Erickson, Kenneth R. Woodard, Lynn E. Sollenberger, Joao M. B. Vendramini, Robert. A. Gilbert +2 others
  • 2013
Carbohydrate and lignin composition of feedstock materials are major factors in determining their bioenergy potential. This study was conducted to quantify dry biomass yield and the carbohydrate and lignin composition of six potential biofuel grasses (elephantgrass, energycane, sweetcane, giant reed, giant miscanthus, and sugarcane) across three sites in(More)
Terpenoids, naturally occurring compounds derived from isoprene units present in pine oleoresin, are a valuable source of chemicals used in solvents, fragrances, flavors, and have shown potential use as a biofuel. This paper describes a method to extract and analyze the terpenoids present in loblolly pine saplings and pine lighter wood. Various extraction(More)
Cortical microtubules are integral to plant morphogenesis, cell wall synthesis, and stomatal behaviour, presumably by governing cellulose microfibril orientation. Genetic manipulation of tubulins often leads to abnormal plant development, making it difficult to probe additional roles of cortical microtubules in cell wall biogenesis. Here, it is shown that(More)
Forest tree breeders often use single-tree plots, rather than multiple-tree plots, to maximize the number of genotypes tested, increase precision of genetic predictions, and achieve greater genetic gains per unit of time. However, genetically improved individuals are deployed operationally on large tracts of land, as stands, more like multiple-tree plots.(More)
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