Garth B. Robinson

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The effects of salts and non-ionic detergents on renal brush borders have been studied. 2 M sodium chloride, iodide or thiocyanate dissociated up to 40% of the protein from the brush borders, destroying the core filaments and resulting in the formation of membrane vesicles; EDTA had a similar effect on structure but released little protein. Triton X-100 and(More)
Cross-linking glomerular basement membrane (GBM) has been shown to render it more permeable to protein. Isolated pig GBM was cross-linked with dimethylmalonimidate which reacts selectively with lysine epsilon-NH2 groups or with glutaraldehyde, a less selective cross-linking agent. Studies of the ultrafiltration properties of these materials in vitro using(More)
The permeability of glomerular basement membrane (GBM) was assessed in vitro by the filtration of solutions of proteins across films formed from isolated pig GBM. Incubation of the films with fructose or glucose increased their permeability to water and serum albumin. The effect of fructose was similar to that previously noted for films crosslinked with(More)
Granules were isolated from both human and rabbit polymorphonuclear leukocytes (PMNs), and the quantity and solubility of certain granule enzymes compared. Lysozyme andα-mannosidase were found to be the most soluble enzymes in the granules of both species and could be released by lysis with 0.1 % (v/v) Triton X-100. Alkaline phosphatase was also released by(More)
The filtration of differently charged species of myoglobin and dextran across films of isolated basement membrane in vitro showed that the filtration behavior of both polymers was influenced by charge. Rejection increased with increasing negative charge. Titration of the isolated basement membrane revealed an isoionic point of pH 5.5 and an isoelectric(More)
Earlier studies indicated that chemically crosslinking glomerular basement membrane (GBM) rendered it more permeable to water and to macromolecules. Here possible mechanisms for the introduction of crosslinks into GBM under pathological conditions were explored. Glycation with glucose and with fructose over periods of 2 wk (fructose) and 6 weeks (glucose)(More)
The ultrafiltration properties of isolated glomerular basement membrane were studied in vitro by forming membrane fragments into thin films for use as ultrafiltration membranes. The filtration properties of the films were examined using cytochrome c, myoglobin, lysozyme, ovalbumin, lactoglobulin, and serum albumin. The films behaved as compressible filters(More)
The presence of carbohydrate in many plasma and tissue proteins is well established (1, 2). The glycoproteins thus far studied contain one or several oligosaccharide units linked to a peptide chain by uncharacterized linkages. Little is yet known about the biosynthetic processes by which the oligosaccharide units are assembled and attached to the(More)