Gareth Girling

Learn More
Human parechoviruses (HPeVs) are frequent pathogens with a seroprevalence of over 90% in adults. Recent studies on these viruses have increased the number of HPeV types to eight. Here we analyse the complete genome of one clinical isolate, PicoBank/HPeV1/a, and VP1 and 3D protein sequences of PicoBank/HPeV6/a, isolated from the same individual 13 months(More)
The genomes of malaria parasites contain many genes of unknown function. To assist drug development through the identification of essential genes and pathways, we have measured competitive growth rates in mice of 2,578 barcoded Plasmodium berghei knockout mutants, representing >50% of the genome, and created a phenotype database. At a single stage of its(More)
The effect of a combination low sodium, low fat, high fibre diet on serum lipids in 184 treated hypertensive patients was analysed in a controlled trial. The combination diet caused a small but significant reduction in serum triglyceride and total cholesterol. This was apparently due to the low fat component of the diet, since a low fat diet alone also(More)
The genome-wide identification of gene functions in malaria parasites is hampered by a lack of reverse genetic screening methods. We present a large-scale resource of barcoded vectors with long homology arms for effective modification of the Plasmodium berghei genome. Cotransfecting dozens of vectors into the haploid blood stages creates complex pools of(More)
The Plasmodium Genetic Modification (PlasmoGEM) database ( provides access to a resource of modular, versatile and adaptable vectors for genome modification of Plasmodium spp. parasites. PlasmoGEM currently consists of >2000 plasmids designed to modify the genome of Plasmodium berghei, a malaria parasite of rodents, which can(More)
An 8-week combination low sodium, low fat, high fibre diet was compared to the individual components of this diet in a controlled trial using 193 patients already on treatment for essential hypertension. No significant changes were observed in the high fibre group. The low sodium group showed a decrease in systolic blood pressure which was close to(More)
The effect of a low sodium, low fat, high fibre diet in allowing a reduction of antihypertensive medication was compared with the effect produced by the individual components of this diet in an observer-blind controlled trial using 196 patients with essential hypertension. Patients were followed up 1.5 months after the last change in medication. In the(More)
One of the factors determining drug t r ea tment in hypertension is the metabolic• side effects of the drugs, including the effect on serum lipids. Mos t druginduced changes have rarely been fol lowed for more than a year [1,2], of ten involve small ( < 30) numbers of patients, and f requent ly take little account of any changes in the pat ients ' diets(More)
  • 1