Three-dimensional (3D) reconstruction from serial sections allows identification of objects of interest in 3D and clarifies the relationship among these objects. 3D_Viewer, developed in our laboratory for this purpose, has four major functions: image alignment, movie frame production, movie viewing, and shift-overlay image generation. Color images captured… (More)
Figure 1: A section of a mouse brain. The overall image is 6656x3584, yet the vessels of interest are 2-3 pixels wide.