Gangrong Xie

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Mitochondrially bound rat brain hexokinase was labeled with the photoactivatable reagent, 3-(trifluoromethyl)-3-(m-[125I]iodophenyl)diazirine. This highly hydrophobic reagent is strongly partitioned into the hydrophobic environment of the membrane core, and thus selectively labels segments of a protein that penetrate this region of the membrane. Labeling of(More)
Rat brain hexokinase (ATP:D-hexose-6-phosphotransferase; EC 2.7.1.1) was derivatized with sulfosuccinimidyl-2-(m-azido-o-nitrobenzamido)ethyl-1,3'-dithiopro pionate (SAND), a photosensitive and cleavable crosslinking agent. The catalytic activity and mitochondrial binding properties of the enzyme were only marginally affected by reaction with SAND. When the(More)
The presence of redundant genes in a genome might enable bacteria to adapt to varying environmental conditions. The genus Gordonia comprises metabolically diverse bacteria that can degrade a wide range of persistent compounds. This paper reports the multiplicity of genes encoding 3-ketosteroid Δ1-dehydrogenase (KstD) in Gordonia neofelifaecis NRRL B-59395.(More)
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