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This study deals with an effective nucleic acids extraction method from various strains of Botryococcus braunii which possesses an extensive extracellular matrix. A method combining freeze/thaw and bead-beating with heterogeneous diameter of silica/zirconia beads was optimized to isolate DNA and RNA from microalgae, especially from B. braunii. Eukaryotic(More)
BACKGROUND Microalgae are considered promising alternative energy sources because they consume CO2 and accumulate large amounts of lipids that can be used as biofuel. Nannochloropsis is a particularly promising microalga due to its high growth rate and lipid content, and the availability of genomic information. Transcription factors (TFs) are global(More)
Oleaginous microalgae of the Nannochloropsis genus are considered excellent candidates for biofuels and value-added products owing to their high biomass productivity and lipid content. Here, we report the first overexpression and detection of a heterologous sfCherry fluorescent protein in Nannochloropsis salina in order to develop a transformation toolbox(More)
Spirulina platensis, a filamentous cyanobacterium, produces γ-linolenic acid (GLA, 18:3), which is an important anti-inflammatory for pharmaceutical use. Thus, to increase the GLA content in S. platensis, this study investigated the combined effect of a light–dark (LD) two-stage culture and mixotrophic culture including a precursor of GLA. When compared(More)
An alternative stress-inducing method, a low-energy pulsed electric field (LE-PEF), was used to improve the lipid productivity of microalgae cultures. A large shift in the Nile-Red-stained peaks toward a higher intensity in fluorescent-activated cell sorting, suggestive of an increased neutral lipid content, was observed when 10 kV LE-PEF pulses were(More)
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