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The aim of this paper was to study the chemical composition of the precipitates found in the intestine of Dicentrarchus labrax and the source of HCO3 − secreted into the intestinal lumen. The chemical analysis was performed by employing the potentiometric double titration method and by means of an electron microscope coupled with a spectrometer and X-ray(More)
Quantitative parameters for the interactions between phytate (Phy) and Al(3+), Fe(3+), and Cr(3+) were determined potentiometrically in NaNO(3) aqueous solutions at I = 0.10 mol L(-1) and T = 298.15 K. Different complex species were found in a wide pH range. The various species are partially protonated, depending on the pH in which they are formed, and are(More)
The acid–base properties of γ-l-glutamyl-l-cysteinyl-glycine (glutathione, GSH) were determined by potentiometry (ISE-H+, glass electrode) in pure NaI(aq) and in NaCl(aq)/MgCl2(aq), and NaCl(aq)/CaCl2(aq) mixtures, at T = 298.15 K and different ionic strengths (up to I c  ~ 5.0 mol L−1). In addition, the activity coefficients of glutathione were also(More)
Alkali metal ions play very important roles in all biological systems, some of them are essential for life. Their concentration depends on several physiological factors and is very variable. For example, sodium concentrations in human fluids vary from quite low (e.g., 8.2 mmol dm(-3) in mature maternal milk) to high values (0.14 mol dm(-3) in blood plasma).(More)
Ethylenediamine-N,N'-disuccinic acid is a biodegradable alternative to EDTA, therefore its use for the sequestration of Ca(2+), Sn(2+), Cu(2+), Zn(2+) and Fe(3+) is analyzed. New data on its binding ability towards these cations were obtained with potentiometric, voltammetric and calorimetric measurements at different ionic strengths and at T = 298.15 K.(More)
The antigenicity of peptide fragments derived from CNBr-cleaved sperm whale Mb has been tested in competitive inhibition assays. These peptides appeared to be very poor inhibitors in a reaction involving radiolabeled sperm whale Mb with antipeptide antibodies, requiring about 10(4)-fold excess of peptides to achieve the same level of inhibition as cold(More)
New potentiometric experiments have been performed in NaCl and in (CH3)4NCl media, to determine the protonation constants, the protonation enthalpy changes and the solubility of six natural α-amino acids, namely Glycine (Gly), Alanine (Ala), Valine (Val), Leucine (Leu), Serine (Ser) and Phenylalanine (Phe). The aim of the work is the rationalization of the(More)
We have resolved anti-sperm whale Mb antibodies into two distinct populations: One population is reactive only with the native molecule, and the second is reaction with both Mb and peptide fragments. CNBr cleavage of sperm whale Mb yields three peptides: peptide I (1-55), peptide II (56-131), and peptide III (132-153). Immunoadsorbent columns made with(More)
Thermodynamic information about the metal-ligand interaction between Fe3+, Zn2+, Cu2+ and Sn2+, and a biodegradable ligand as MGDA is reported. The speciation scheme was obtained by means of potentiometric measurements and isothermal titration calorimetry (to determine enthalpy changes) in NaCl medium. The formation of the ML and MLOH species was evidenced(More)