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The present work reviews the main advancements achieved in the last decades in the study of the fructose production process by inulin enzymatic hydrolysis. With the aim of collecting and clarifying the majority of the knowledge in this area, the research on this subject has been divided in three main parts: a) the characteristics of inulin (the process(More)
1. An analysis of the kinetic behaviour of immobilized acid phosphatase (EC 3.1.3.2) layers, gelled on the active surface of an ultrafiltration membrane, was carried out. 2. Two possible forms of such immobilized-enzyme systems were dealt with, namely enzyme-polyalbumin co-gelation through an ultrafiltration process, and enzyme co-polymerization to the same(More)
A central composite design (CCD) was performed to evaluate the effects of four factors, i.e. temperature (T), pH, agitation rate (K) and initial lactose concentration (L), on ricotta cheese whey batch fermentation and to optimize the process leading to the formation of bio-ethanol. Anaerobic batch fermentation experiments were carried out by using the yeast(More)
In the present paper a factor analysis is presented for the enzymatic transesterification of waste oil for biodiesel production. The experimental data on batch reactor evidence two key variables: enzyme loading and mixing conditions. These variables were subjected to a factor analysis and their combined effect on the reaction performance was determined.(More)
The present work is an experimental study of the performance of a recently designed immobilized enzyme: inulinase from Aspergillus sp. covalently immobilized on Sepabeads. The aim of the work is to test the new biocatalyst in conditions of industrial interest and to assess the feasibility of the process in a fluidized bed bioreactor (FBBR). The catalyst was(More)
In this paper, the reaction of enzymatic trans-esterification of glycerides with ethanol in a reaction medium containing hexane at a temperature of 37 degrees C has been studied. The enzyme was Lipase from Mucor miehei, immobilized on ionic exchange resin, aimed at achieving high catalytic specific surface and recovering, regenerating and reusing the(More)
The present paper was aimed at showing that advanced modeling techniques, based either on artificial neural networks or on hybrid systems, might efficiently predict the behavior of two biotechnological processes designed for the obtainment of second-generation biofuels from waste biomasses. In particular, the enzymatic transesterification of waste-oil(More)
In this work, the fermentation of " Ricotta cheese whey " for the production of ethanol was simulated by means of a Hybrid Neural Model (HNM), obtained by coupling neural network approach to mass balance equations describing the time evolution of lactose (substrate), ethanol (product) and biomass concentrations. The realized HNM was compared with a pure(More)
Both immobilization techniques described in this work seem appropriate for this allosteric enzyme. However, the kinetic behavior of the dCMP deaminase trapped in a protein membrane is markedly different from that observed with the enzyme in solution and with the enzyme gelled on an artificial membrane. The enzyme, in fact, in all instances loses its(More)