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Replication intermediates that escape Dna2 activity are processed by Holliday junction resolvase Yen1
This work finds the Dna2 helicase activity acts parallel to homologous recombination in promoting DNA replication and chromosome detachment at mitosis after replication fork stalling, and identifies a non-canonical role for Yen1 in the processing of replication intermediates that is distinct from HJ resolution.
Rif1 maintains telomeres and mediates DNA repair by encasing DNA ends
Crystal structures for the uncharacterized and conserved ∼125-kDa N-terminal domain of Rif1 from Saccharomyces cerevisiae (Rif1-NTD) are described, revealing an α-helical fold shaped like a shepherd's crook that fully encases DNA as a head-to-tail dimer in DNA repair.
Mechanisms of replication and repair in mitochondrial DNA deletion formation
The role of replication and repair in mtDNA deletion formation as well as nucleic acid motifs such as repeats, secondary structures, and DNA damage associated with deletion formation in the mitochondrial genome are discussed.
Oxidative stress controls the choice of alternative last exons via a Brahma–BRCA1–CstF pathway
It is shown that Brahma (BRM), the ATPase sub unit of the hSWI/SNF chromatin-remodeling complex interacts with BRCA1/BARD1, which ubiquitinates the 50 kDa subunit of the 3′ end processing factor CstF, which results in the inhibition of transcript cleavage at the proximal poly(A) site and a shift towards inclusion of the distal terminal exon.
Rif1 S-acylation mediates DNA double-strand break repair at the inner nuclear membrane
Rif1 S-acylation facilitates the accumulation of Rif1 at DSBs, attenuation of DNA end-resection, and DSB repair by non-homologous end-joining by the DHHC family palmitoyl acyltransferase Pfa4.
Shepherding DNA ends: Rif1 protects telomeres and chromosome breaks
An overview of the actions of RIF1 at DNA ends is provided and how exclusion of end-processing factors might be the underlying principle allowing Rif1 to fulfill diverse biological roles at telomeres and chromosome breaks is explored.
Compartmentalized DNA repair: Rif1 S-acylation links DNA double-strand break repair to the nuclear membrane
Findings reveal S-fatty acylation and membrane anchorage of Rap1-interacting factor 1 (Rif1) as a mechanism providing spatial control over DSB repair pathway choice.
An epigenetic view on alternative splicing
This review will focus on how the modulation of the transcribing RNA polymerase II, the changes in nucleosome architecture and the presence of different histone modifications contribute to the regulation of the splicing process.
Chapter 4 An Epigenetic View on Alternative Splicing
Genome-wide analysis indicate that alternative splicing of mRNA precursors (premRNAs) affects the vast majority of human genes. Alternative splicing provides a fundamental mechanism to increase