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Human epidermal growth factor ͑hEGF͒ was expressed in Nicotiana plants using integrative and non-integrative systems. Nuclear transformation was carried out using genetic constructs that allow hEGF accumulation into the cytoplasmic or apoplastic spaces. Protein level did not exceed 0.00001% of total soluble protein in the cyto-plasm, but reached values of(More)
BACKGROUND Modified Vaccinia Ankara (MVA) is an attenuated strain of Vaccinia virus (VACV) currently employed in many clinical trials against HIV/AIDS and other diseases. MVA still retains genes involved in host immune response evasion, enabling its optimization by removing some of them. The aim of this study was to evaluate cellular immune responses (CIR)(More)
A recombinant modified vaccinia Ankara (MVA) virus expressing mature viral protein 2 (VP2) of the infectious bursal disease virus (IBDV) was constructed to develop MVA-based vaccines for poultry. We demonstrated that this recombinant virus was able to induce a specific immune response by observing the production of anti-IBDV-seroneutralizing antibodies in(More)
MVA is an attenuated vector that still retains immunomodulatory genes. We have previously reported its optimization after deleting the C12L gene, coding for the IL-18 binding-protein. Here, we analyzed the immunogenicity of MVA vectors harboring the simultaneous deletion of A44L, related to steroid synthesis and A46R, a TLR-signaling inhibitor(More)
Infectious bursal disease virus (IBDV) causes infectious bursal disease, a highly contagious immunosuppressive disease that affects young chickens and causes economic losses in the poultry industry worldwide. IBDV replic-ates mainly in actively dividing B lymphocytes within the bursa of Fabricius (BF), leading to immunosuppres-sion in affected flocks(More)
Orf virus (ORFV) is the etiological agent of contagious ecthyma (CE), a pustular dermatitis of sheep and goats. Outbreaks of ORFV have been observed in all geographical regions of the world, including Argentina. The origin and identity of Argentinian ORFVs are unknown, and no comparative or phylogenetic studies of these viruses have been performed. In this(More)
Canarypox viruses (CNPV) carrying the coding sequence of VP2 protein from infectious bursal disease virus (IBDV) were obtained. These viruses were able to express VP2 protein in vitro and to induce IBDV-neutralizing antibodies when inoculated in specific pathogen-free chickens demonstrating that CNPV platform is usefulness to develop immunogens for chickens.
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