Gabriela Bernal

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The conceptual bases of recent NRC publications of nutrient requirements to meet dietary energy needs of growing Holsteins, emphasizing heifers gaining 0.75 kg/d, are described and compared with several world systems and with body composition data. Requirements of Holsteins for net energy for maintenance are generally thought to be higher (from 7 to 20%)(More)
Though in vitro culture of primary intestinal organoids has gained significant momentum in recent years, little has been done to investigate the impact of microenvironmental cues provided by the encapsulating matrix on the growth and development of these fragile cultures. In this work, the impact of various in vitro culture parameters on primary adult(More)
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