PURPOSE To compare the outcome of corneal grafts preserved in McCarey-Kaufman (MK) medium versus organ culture after penetrating keratoplasties. METHODS Paired corneas were stored in McCarey medium for 2-44 hours (mean 21 hours) and in organ culture (OC) for 144 -240 hours (mean 192 hours). Penetrating keratoplasties were performed by 2 surgeons in 2… (More)
PURPOSE The drug dichloromethylene diphosphonate (CL2MDP) encapsulated in liposomes depletes macrophages but not other immunocompetent cells. The authors investigated whether subconjunctival injection of CL2MDP containing liposomes (CL2MDP-LIP) could prolong survival of corneal allografts in rats. METHODS Male Fisher rats received orthotopic corneal… (More)
PURPOSE To compare two different optotypes to measure visual acuity. METHODS Experiment 1: Fifty patients with moderate cataracts were asked to read a chart consisting of letters of the alphabet (Sloan letters) first and a chart comprising Landolt's broken rings afterwards. Experiment 2: Half of patients were instructed to repeat the reading with a second… (More)
Twenty-eight paired human corneas were preserved in minimal essential medium at 31°C and in McCarey-Kaufman medium at 4°C. These grafts were then transplanted in pairs of patients with keratoconus who were age matched as closely as possible. These pairs received donor corneas from the same donor, so for each pair the donor age and time from death to… (More)
PURPOSE In rats, corneal allograft rejection is delayed for at least 100 days by clodronate liposomes. These liposomes selectively deplete macrophages. To investigate the immunologic basis for absence of graft rejection in treated rats, the effect of these liposomes on the generation of cytotoxic T lymphocytes (CTLs) and antibody production after orthotopic… (More)
Association between corneal allograft reactions and HLA compatibility.
PURPOSE To determine the effect of a fresh corneal wound or a healed corneal scar on the immunodiffusion of immunoglobulins into the cornea. METHODS F344 rats were immunized with human serum albumin (HSA) 1 week before an autologous rotational keratoplasty of the right cornea or 1 year after an autograft was performed. One group of rats also was treated… (More)