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Resistance to Plasmodium chabaudi has been examined in different inbred mouse strains bearing identical H-2 haplotypes on different genetic backgrounds as well as in H-2-congenic mouse strains on B10 background. Resistance is expressed in terms of percent survival after a challenge with 10(6) P. chabaudi-infected erythrocytes. We can show that murine(More)
Erythrocytes infected with the malaria parasite Plasmodium chabaudi contain the neo-protein Pc90 in their plasma membrane. We investigate origin, membrane disposition, and intraerythrocytic traffic of this Pc90. Metabolic labeling of P.-infected erythrocytes, combined with cell fractionation as well as Western blot analysis and immunoprecipitation using a(More)
Plasma membranes of Plasmodium chabaudi-infected erythrocytes contain seven major neoproteins with apparent molecular masses of 154, 145, 90, 72, 67, 52, and 33 kDa, respectively. These neoproteins, with the exception of the two larger ones, can be metabolically labelled with [14C]isoleucine. The seven neoproteins are antigenic as revealed by Western(More)
A new procedure has been developed which allows the concomitant isolation of viable parasites and host cell plasma membranes from erythrocytes infected with Plasmodium chabaudi trophozoites. The average final yield of parasites is 56%. Free parasites reveal a well preserved ultrastructure, incorporate [14C]isoleucine for at least 3 h, and synthesize about(More)
We have developed a new procedure for subfractionation ofPlasmodium chabaudi-infected erythrocytes into parasites and ghosts. Trophozoite-infected erythrocytes enriched over a percoll-step (ϱ: 1.10 g/cm3) are subjected to a glycerol-enhanced osmotic shock. This induces the release of parasites and the emergence of erythrocyte ghosts, which can be separated(More)
Neoproteins were compared in host cell plasma membranes isolated as ghosts from erythrocytes infected with early and late trophozoites of Plasmodium chabaudi. Ghosts isolated from early trophozoites-infected erythrocytes (= et-ghosts) contain seven neoproteins with apparent molecular masses of about 154, 145, 90, 72, 67, 52 and 33 kDa, respectively. Two(More)