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1. A lactating-sheep mammary gland was perfused for 12h in the presence of l-[2-(14)C]-citrulline and received adequate quantities of glucose, acetate and amino acids. Two lactating-goat udders were similarly perfused in the presence of either l-[carbamoyl-(14)C,-2-(14)C]citrulline or l-[carbamoyl-(14)C,1-(14)C]citrulline and l-[4-(3)H]arginine. 2. In these(More)
The effect of intramammary infusion of Escherichia coli endotoxin upon mammary blood flow was studied in lactating goats and cows. Blood flow was recorded by means of an electromagnetic flow probe chronically implanted around one mammary artery. Endotoxin mastatis was always accompanied by a significant increase in mammary blood flow, characterized by 2(More)
The serum growth hormone (BGH) and free fatty acid (FFA) concentrations of large groups of calves (male and female ranging in age from 8 to 14 days and from 3 to 6 months old), bulls, oxen, heifers and cows under normal conditions were compared with the serum BGH and FFA values of corresponding large groups of animals under stress conditions. Stress was(More)
beta-Adrenoceptors present in smooth muscles of the cistern wall of teats of lactating cows were identified and characterized by 3H-dihydroalprenolol (DHA) binding. Binding association and dissociation at 37 degrees C were very rapid (t1/2 amounting to 2 min and 1 min 30 sec respectively), stereospecific and saturable. By Scatchard analysis, the mean(More)
Mammary blood flow (MBF) was recorded chronically in lactating goats by means of an electromagnetic flow probe. Experimental mastitis was induced by intramammary injection of E. coli lipopolysaccharides. Control mastitis experiments were accompanied by a significant increase in MBF characterized by 2 conspicuous peaks, by fever, increased heart rate,(More)
Four lactating goat mammary glands were perfused for several hours in the presence of ornithine labeled with 1-carbon-14 and delta-nitrogen-15 or 5-hydrogen-3 and received adequate quantities of glucose, acetate, and amino acids. For the first label carbon and nitrogen were incorporated in casein of milk. After isolation of the casein amino acids the(More)
The influence of intravenous injections or infusions of insulin (0-2 i.u./kg), propranolol (150 mg), 3,5-dimethylpyrazole (3 mg/kg), 3,5-dimethylisoxazole (0-08 mg/kg), glucose (0-5 g/kg), nicotinic acid (120 mg/kg), arginine (0-5 g/kg) or butyrate (0-5 mmol/kg) on plasma glucose levels, and on serum concentrations of growth hormone and free fatty acids of(More)
A 22-year-old woman is described with gastrointestinal complaints, sclerokeratitis, and a bilateral progressive hearing loss. The clinical picture, the outcome of lymphocyte stimulation tests against S antigen (retina soluble antigen), outer rod segment, and scleroprotein, and the successful administration of corticosteroids after a partial spontaneous(More)