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The development of gamma-aminobutyric acid-immunoreactivity (GABA-I) in nucleus magnocellularis (NM) and nucleus laminaris (NL) of the chick was studied by using an antiserum to GABA. In posthatch chicks, GABA-I is localized to small, round punctate structures in the neuropil and surrounding nerve cell bodies. Electron microscopic immunocytochemistry(More)
Development of the olfactory epithelia of the African clawed frog, Xenopus laevis, was studied by scanning and transmission electron microscopy. Stages examined ranged from hatching through the end of metamorphosis. The larval olfactory organ consists of two chambers, the principal cavity and the vomeronasal organ (VNO). A third sensory chamber, the middle(More)
The fine structure of synaptic terminals contacting neurons generated in the forebrain of adult male canaries was investigated by autoradiography and electron microscopy. The procedure for labeling the new neurons included pretreating adult canaries with 3H-thymidine and sacrificing them 23-45 days later. Neurons were identified as newly generated by the(More)
In adult Xenopus, the nasal cavity is divided into separate middle (MC) and principal (PC) cavities; the former is used to smell water-borne odorants, the latter air-borne odorants. Recent work has shown that olfactory neurons of each cavity express a distinct subclass of odorant receptors. Moreover, MC and PC axons project to distinct regions of the(More)
Development of the olfactory system in Xenopus laevis begins during gastrulation, with the induction of olfactory placodes at the rostral edge of the prospective neural plate. Initial placodal induction appears to involve cerberus, a molecule secreted from the involuting anterior endoderm. Possible downstream genes expressed in the anterior neural ridge and(More)
Combinations of the Golgi stain, anterograde degeneration, and electron microscopy are used to further characterize the hormone-sensitive "type IV" neuron of the forebrain nucleus robustus archistriatalis (RA) of adult female canaries. Anterograde degeneration was used to "stain," at the electron-microscopic level, the axon terminals of neurons projecting(More)
Experiments were conducted to examine by light and electron microscopy the localization of acetylcholinesterase (AChE) in the main (MOB) and accessory (AOB) olfactory bulbs of the normal mouse. Evidence from the literature for cholinergic innervation of the mammalian olfactory bulb was then assessed in light of possible correlation between reported sites of(More)
We determined the time of origin of neurons in the olfactory bulb of the South African clawed frog, Xenopus laevis. Tritiated thymidine injections were administered to frog embryos and tadpoles from gastrulation (stage 11/12) through metamorphosis (stage 65), paraffin sections were processed for autoradiography, and the distribution of heavily and lightly(More)
  • G D Burd
  • Microscopy research and technique
  • 1993
The effects of intranasal zinc sulfate (ZnSO4) irrigation on the morphology of the olfactory epithelium and olfactory bulb were studied in mice with short survival times (as early as 1 day) and with long survival times (up to 593 days) after the irrigation procedure. As in several previous studies, the olfactory epithelium was completely destroyed within a(More)
The relationship between olfactory axons and the cells of the olfactory bulb during normal development was analyzed to determine whether olfactory afferent axons could play a role in the induction of olfactory bulb formation. The morphology of the olfactory bulb in Xenopus larvae from stages 26 to 58 and in adult frogs was analyzed with light and electron(More)