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Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa.
A discontinuous sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) system for the separation of proteins in the range from 1 to 100 kDa is described, and the omission of glycine and urea prevents disturbances which might occur in the course of subsequent amino acid sequencing. Expand
Analysis of molecular masses and oligomeric states of protein complexes by blue native electrophoresis and isolation of membrane protein complexes by two-dimensional native electrophoresis.
A two-dimensional native technique was developed that is suitable for preparation of highly pure membrane protein complexes and was applied to analysis of cytochrome bc/bf complexes. Expand
Blue native electrophoresis for isolation of membrane protein complexes in enzymatically active form.
The percentage recovery of functional activity depended on the respective protein complex studied and was zero for some complexes, but almost quantitative for others, and the recovery of all respiratory chain complexes was almost quantitative. Expand
Coomassie blue-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for direct visualization of polypeptides during electrophoresis.
A method for the direct visualization of Coomassie blue-stainedpolypeptide bands during electrophoresis with subsequent elution of polypeptides and removal of sodium dodecyl sulfate and CoomASSie blue is described to allow the preparative separation of protein fragments. Expand
Zinc Ions Inhibit the Qp Center of Bovine Heart Mitochondrial bc1 Complex by Blocking a Protonatable Group (*)
Zinc binds 3 times tighter to Fe-S-depleted bc1 complex indicating that the zinc binding site is not on the “Rieske” iron-sulfur protein in contrast to a recent report by Lorusso et al. Expand
Pathways of hydrogen in mitochondria of Saccharomyces carlsbergensis.
With mitochondria from Saccharomyces carlsbergensis the functional relationship of the exogenous and endogenous NAD system has been investigated with respect to the membrane barrier, the localisation of NADH dehydrogenase and other NAD-and flavine-linked dehydrogenases and the existence of a mitochondrial alcohol dehydrogensase is established. Expand
The molecular basis for the natural resistance of the cytochrome bc1 complex from strobilurin-producing basidiomycetes to center Qp inhibitors.
The exon/intron organization was determined and the sequences of cytochromes b from S. tenacellus, M. galopoda and a third basidiomycete, Mycena viridimarginata, which produces no strobilurin A were deduced to be naturally resistant to (E)-beta-methoxyacrylates. Expand
Oudemansin, strobilurin A, strobilurin B and myxothiazol: new inhibitors of the bc 1 segment of the respiratory chain with an E‐β‐methoxyacrylate system as common structural element
The inhibitory action and binding characteristics of the new anti-fungal antibiotic myxothiazol has been described and a red shift of the ferrocytochrome b spectrum is indicated, independent of and differs from the antimycin red shift. Expand
Ubiquinol-cytochrome-c reductase from human and bovine mitochondria.
This chapter concludes with the discussion on the structure–function relationship of quinone reaction centers. Expand
Point mutation in cytochrome b of yeast ubihydroquinone:cytochrome-c oxidoreductase causing myxothiazol resistance and facilitated dissociation of the iron-sulfur subunit.
- B. M. Geier, H. Schägger, U. Brandt, A. Colson, G. von Jagow
- Biology, Medicine
- European journal of biochemistry
- 1 September 1992
This is the first report of a single amino acid exchange in the catalytic subunit of cytochrome b, greatly affecting the iron-sulfur protein, the second important catalyticSubunit of the Q0 center. Expand