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Loss of membrane cholesterol influences lysosomal permeability to potassium ions and protons.
Cholesterol is an essential component of lysosomal membranes. In this study, we investigated the effects of membrane cholesterol on the permeability of rat liver lysosomes to K+ and H+, and theExpand
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  • Open Access
Mechanism of Lysophosphatidylcholine-Induced Lysosome Destabilization
Lysosomal destabilization is critical for the organelle and living cells. Phospholipase A2 (PLA2) was shown to be able to destabilize lysosomes under some conditions. By what mechanism the enzymeExpand
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  • Open Access
Loss of membrane cholesterol affects lysosomal osmotic stability.
Lysosomal destabilization is critical for the organelle and living cells. Using methyl-beta-cyclodextrin (M beta CD) to selectively deplete lysosomal membrane cholesterol, we investigated the effectExpand
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The hydrolysis of cell surface glycosphingolipids by endoglycoceramidase reduces epidermal growth factor receptor phosphorylation in A431 cells.
This paper presents a new method to evaluate the biological significance of glycosphingolipids (GSLs) using a GSL-specific enzyme, endoglycoceramidase (EGCase), by which GSL-sugar chains are removedExpand
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Effects of Arachidonic Acid on the Lysosomal Ion Permeability and Osmotic Stability
In this study, we investigated the effects of arachidonic acid, a PLA2-produced lipid metabolite, on the lysosomal permeability, osmotic sensitivity and stability. Through the measurements ofExpand
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  • Open Access
Lysosome destabilization by cytosolic extracts, putative involvement of Ca2+/phospholipase C
Lysosomal disintegration is a crucial event for living cells, but mechanisms for the event are still unclear. In this study, we established that the cytosolic extracts could enhance lysosomal osmoticExpand
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  • Open Access
Influence of membrane physical state on lysosomal potassium ion permeability
Lysosomal permeability to potassium ions is an important property of the organelle. Influence of the membrane physical state on the potassium ion permeability of isolated lysosomes was assessed byExpand
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  • Open Access
Mechanism of cytosol phospholipase C and sphingomyelinase-induced lysosome destabilization.
Lysosomal disintegration may cause apoptosis, necrosis and some diseases. However, mechanisms for these events are still unclear. In this study, we measured lysosomal beta-hexosaminidase freeExpand
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  • Open Access
Influence of the physical states of membrane surface area and center area on lysosomal proton permeability.
The physical state of the lysosomal membrane was modulated with the membrane fluidizers n-propanol and n-octanol and with the membrane rigidifiers cholesteryl hemisuccinate and cholesterol. MembraneExpand
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Enhancement of Lysosomal Osmotic Sensitivity Induced by the Photooxidation of Membrane Thiol Groups¶
Abstract The osmotic lysis of photodamaged lysosomes is a critical event for killing tumor cells. How the photodamage increases lysosomal osmotic sensitivity is still unclear. In this work, theExpand
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