The Cell Adhesion Protein P-selectin Glycoprotein Ligand-1 Is a Substrate for the Aspartyl Protease BACE1*
- S. Lichtenthaler, D. Dominguez, B. Seed
- Biology, ChemistryJournal of Biological Chemistry
- 5 December 2003
It is shown that BACE1 acts on the P-selectin glycoprotein ligand 1 (PSGL-1), which mediates leukocyte adhesion in inflammatory reactions, and concludes that PS GL-1 is an additional substrate for Bace1.
A non‐amyloidogenic function of BACE‐2 in the secretory pathway
- R. Fluhrer, A. Capell, J. Walter
- BiologyJournal of Neurochemistry
- 1 June 2002
Radiosequencing of the membrane‐bound C‐terminal cleavage product revealed that BACE‐2 cleaves βAPP in the middle of the Aβ domain between phenylalanines 19 and 20, resulting in increased secretion of APPs‐α‐ and p3‐like products and reduced production of Aβ species.
Identification of a β-Secretase Activity, Which Truncates Amyloid β-Peptide after Its Presenilin-dependent Generation*
- R. Fluhrer, G. Multhaup, C. Haass
- Biology, ChemistryJournal of Biological Chemistry
- 21 February 2003
It is demonstrated that an apparently γ-secretase-dependent Aβ derivative is produced after the generation of the non-truncated Aβ via an additional and unexpected activity of BACE.
Dimerization of beta-site beta-amyloid precursor protein-cleaving enzyme.
- G. Westmeyer, M. Willem, C. Haass
- BiologyJournal of Biological Chemistry
- 2004
It is demonstrated that dimerization of BACE occurs already before full maturation and pro-peptide cleavage, which suggests that under native conditions BACE exists as a homodimer.
A secreted enzyme reporter system for MRI.
- G. Westmeyer, Y. Durocher, A. Jasanoff
- BiologyAngewandte Chemie
- 1 April 2010
This work describes a new reporter gene system for MRI that relieves the need to deliver MRI probes to their sites of action within cells by harnessing an extracellular enzyme, the mammalian secreted alkaline phosphatase (SEAP).
Microfluidic sorting of intrinsically magnetic cells under visual control
- Ahne Myklatun, Michele Cappetta, M. Winklhofer, V. Ntziachristos, G. Westmeyer
- BiologyScientific Reports
- 31 July 2017
A robust and tunable microfluidic sorting system in which magnetic gradients of up to 1700 T/m can be applied to cells flowing through a sorting channel by reversible magnetization of ferrofluids, which ensures contamination-free separation of naturally occurring or bioengineered magnetic cells and is essential for downstream characterization of their properties.
Artifact-free deconvolution in light field microscopy.
- Anca Stefanoiu, Josue Page, Panagiotis Symvoulidis, G. Westmeyer, Tobias Lasser
- Computer ScienceOptics Express
- 28 October 2019
This work analyzes the sampling patterns of the LFM, and introduces a flexible light field point spread function model (LFPSF) to cope with arbitrary LFM designs, and proposes a novel aliasing-aware deconvolution scheme to address the sampling artifacts.
Directed evolution of a magnetic resonance imaging contrast agent for noninvasive imaging of dopamine
- M. Shapiro, G. Westmeyer, A. Jasanoff
- BiologyNature Biotechnology
- 1 March 2010
The results demonstrate the feasibility of molecular-level functional MRI using neural activity–dependent sensors, and the protein engineering approach can be generalized to create probes for other targets.
Calcium Sensor for Photoacoustic Imaging.
- S. Roberts, M. Seeger, G. Westmeyer
- BiologyJournal of the American Chemical Society
- 16 October 2017
We introduce a selective and cell-permeable calcium sensor for photoacoustics (CaSPA), a versatile imaging technique that allows for fast volumetric mapping of photoabsorbing molecules with deep…
Dimerization of β-Site β-Amyloid Precursor Protein-cleaving Enzyme*
- G. Westmeyer, M. Willem, C. Haass
- Biology, ChemistryJournal of Biological Chemistry
- 17 December 2004
It is demonstrated that dimerization of BACE occurs already before full maturation and pro-peptide cleavage, and the purified native BACE dimer revealed a higher affinity and turnover rate in comparison to the monomeric soluble BACE.
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