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Variations of Bacterial Populations in Human Feces Measured by Fluorescent In Situ Hybridization with Group-Specific 16S rRNA-Targeted Oligonucleotide Probes
TLDR
Six 16S rRNA-targeted oligonucleotide probes were designed, validated, and used to quantify predominant groups of anaerobic bacteria in human fecal samples, indicating that normal biological variations within the fecal populations of the volunteers were determined and indicated that these variations should be considered when evaluating the effects of agents modulating the flora.
Analysis of intestinal flora development in breast-fed and formula-fed infants by using molecular identification and detection methods.
TLDR
Novel molecular identification methods were used to verify the data obtained by traditional culture methods and to validate the culture independent fluorescent in situ hybridization (FISH) technique, confirming the differences in development of intestinal flora between breast-fed and formula-fed infants.
Quantitative fluorescence in situ hybridization of Bifidobacterium spp. with genus-specific 16S rRNA-targeted probes and its application in fecal samples
TLDR
Since the total culturable counts were only a fraction of the total microscopic counts, the contribution of bifidobacteria to the total intestinal microflora was overestimated by almost 10-fold when cultural methods were used as the sole method for enumeration.
Analysis of the Fecal Microflora of Human Subjects Consuming a Probiotic Product Containing Lactobacillus rhamnosusDR20
TLDR
It is concluded that consumption of the DR20-containing milk product transiently altered the Lactobacillus and enterococcal contents of the feces of the majority of consumers without markedly affecting biochemical or other bacteriological factors.
Extensive Set of 16S rRNA-Based Probes for Detection of Bacteria in Human Feces
TLDR
Seven new 16S rRNA-based oligonucleotide probes for the detection of six groups of anaerobic bacteria in human feces are designed and given more data on the composition of the human gut flora.
A 16S rRNA-targeted Probe for Detection of Lactobacilli and Enterococci in Faecal Samples by Fluorescent In Situ Hybridization
TLDR
A group-specific 16S rRNA-targeted oligonucleotide probe S-G-Lab-0158-a-A20 (Lab158) was designed and validated to quantify species of the phylogenetic group lactobacilli-enterococci and shows that FISH with probe Lab158 is a useful method to enumerate lactob ACilli in faeces and can assist in the identification of lactic acid bacteria grown on plates.
Development of 16S rRNA-Based Probes for theCoriobacterium Group and the Atopobium Cluster and Their Application for Enumeration of Coriobacteriaceaein Human Feces from Volunteers of Different Age
TLDR
Application of 16S rRNA-targeted probes to fecal samples showed that formula- fed infants had higher numbers of Coriobacterium group cells in their feces than breast-fed infants, and it is hypothesized that with the increasing age of a person, the diversity of Atopobium cluster species present in the feces increases.
Analysis of 16S libraries of mouse gastrointestinal microflora reveals a large new group of mouse intestinal bacteria.
TLDR
Total genomic DNA from samples of intact mouse small intestine, large intestine, caecum and faeces was used as template for PCR amplification of 16S rRNA gene sequences with conserved bacterial primers, revealing a new operational taxonomic unit (OTU) belonging to the Cytophaga-Flavobacter-Bacteroides phylum, which the authors named 'mouse intestinal bacteria'.
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