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Culturability of Mycobacterium tuberculosis cells isolated from murine macrophages: a bacterial growth factor promotes recovery.
Results indicate that a proportion of 'unculturable' M. tuberculosis cells obtained from macrophages is either injured or dormant and that these cells may be recovered or resuscitated using Rpf in liquid medium. Expand
Dormant ovoid cells of Mycobacterium tuberculosis are formed in response to gradual external acidification.
This work demonstrates the efficient formation of phase-dark ovoid cells in MTB cultures within 150 days after the onset of stationary phase as a result of cellular metabolism leading to gradual acidification of the external medium. Expand
Finding of the Low Molecular Weight Inhibitors of Resuscitation Promoting Factor Enzymatic and Resuscitation Activity
A novel class of 2-nitrophenylthiocyanates (NPT) compounds are described that inhibit muralytic activity of Rpfs with IC50 1–7 µg/ml, the first example of low molecular weight compounds that inhibit the enzymatic and biological activities of R pf proteins. Expand
Influence of oxidative and nitrosative stress on accumulation of diphosphate intermediates of the non-mevalonate pathway of isoprenoid biosynthesis in corynebacteria and mycobacteria
A possible role of the MEP-pathway of isoprenoid biosynthesis and a role of its central intermediate MEcDP in bacterial response to nitrosative and oxidative stress is discussed. Expand
Role of lipid components in formation and reactivation of Mycobacterium smegmatis “nonculturable” cells
Oleic acid in concentration of 0.05–3 μg/ml exhibited maximal effect, and that allows us to draw a conclusion concerning its signal role in the transition of dormant cells into active state. Expand
Cell-cell interactions during the formation and reactivation of “nonculturable” mycobacteria
This review considers cell-cell communications involved in the formation and reactivation of NC forms of the bacteria M. smegmatis and M. tuberculosis and pays special attention to the secreted Rpf family proteins, which belong to peptidoglycan hydrolases and participate in the resuscitation of NC mycobacteria. Expand
[Localization of gramicidin S on the cytoplasmic membrane of Bacillus brevis and its effect on the activity of membrane enzymes].
It was shown that malate dehydrogenase of isolated membranes of the gramicidin S producer Bacillus brevis var. G.-B. (R.-form) is completely inhibited by the antibiotic (approximately 200 mkg/mg ofExpand
The role of 2-C-Methylerythritol-2,4-cyclopyrophosphate in the resuscitation of the “nonculturable” forms of Mycobacterium smegmatis
The introduction of an additional copy of the ispE gene encoding cytidyl-methyl-erythritol kinase resulted in the emergence of a capacity for spontaneous reactivation of “nonculturable” M. smegmatis cells, indicative of a previously unknown function of MEC, assumed to consist in regulation of the bacterial genome activity. Expand
Reactivation of dormant "non-culturable" Mycobacterium tuberculosis developed in vitro after injection in mice: both the dormancy depth and host genetics influence the outcome.
The results indicate that bacteria which are able to resuscitate spontaneously in liquid medium in vitro started to multiply in organs of infected mice, and that the outcome of such infection strongly depended upon the level of genetic TB susceptibility. Expand
Diagnostic Methods for Detecting Forms and Strains of Helicobacter Pyloriand Evaluation of Its Eradication
Diagnostic methods for detecting forms and strains of Helicobacter pylori isolated from biopsy specimens of gastric mucosa in 28 patients with duodenal ulcers and evaluation of its eradication wereExpand