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First DNA Barcode Reference Library for the Identification of South American Freshwater Fish from the Lower Paraná River
The sequence library presented here can be confidently used as a benchmark for identification of half of the fish species recorded for the Lower Paraná River, as the minimum genetic distance to the nearest neighbour exceeded the maximum intraspecific distance. Expand
Strategic Approach To Produce Low-Cost, Efficient, and Stable Competitive Internal Controls for Detection of RNA Viruses by Use of Reverse Transcription-PCR
The CIC reported here is an essential reagent for HCV screening in blood banks in resource-limited settings and a versatile, low-cost alternative strategy to armored RNA technology that can be adapted for detection or real-time applications of any RNA target. Expand
Evaluation of the genetic diversity of microsatellite markers among four strains of Oreochromis niloticus.
The UFLA strain was genetically characterized for the first time and, because of its unique origin and genetic distinctness, may prove to be an important resource for genetic improvement of Nile tilapia. Expand
Glycosomal bromodomain factor 1 from Trypanosoma cruzi enhances trypomastigote cell infection and intracellular amastigote growth.
The first characterization of Trypanosoma cruzi bromodomain factor 1 (TcBDF1) is reported, which localizes in the glycosomes directed by a PTS2 (peroxisome-targeting signal 2) sequence and is developmentally regulated. Expand
Trypanosoma cruzi bromodomain factor 2 (BDF2) binds to acetylated histones and is accumulated after UV irradiation.
It is shown that one of those, TcBDF2, is expressed in discrete regions inside the nucleus of all the parasite life cycle stages and binds H4 and H2A purified histones from T. cruzi. Expand
Trypanosoma cruzi Bromodomain Factor 3 Binds Acetylated α-Tubulin and Concentrates in the Flagellum during Metacyclogenesis
ABSTRACT Bromodomains are highly conserved acetyl-lysine binding domains found mainly in proteins associated with chromatin and nuclear acetyltransferases. The Trypanosoma cruzi genome encodes atExpand
Permanent Genetic Resources added to Molecular Ecology Resources Database 1 December 2012–31 January 2013
This article documents the addition of 268 microsatellite marker loci to the Molecular Ecology Resources Database. Loci were developed for the following species: Alburnoides bipunctatus, ChamaeropsExpand
Characterization of TcHMGB, a high mobility group B family member protein from Trypanosoma cruzi.
Immunofluorescence and western blot assays showed that TcHMGB is a nuclear protein expressed in all life cycle stages, however, protein levels seem to vary throughout the life cycle, which may be related to previously described changes in heterochromatin distribution and transcription rates. Expand
Isolation and characterization of 20 polymorphic microsatellite loci in the migratory freshwater fish Leporinus obtusidens (Characiformes: Anostomidae) using 454 shotgun pyrosequencing.
Twenty polymorphic microsatellite markers were developed for the Neotropical fish Leporinus obtusidens using a next generation sequencing approach and tested in two other characifomes species,Expand
Assessing Genetic Diversity for a Pre-Breeding Program in Piaractus mesopotamicus by SNPs and SSRs
The results suggest that the basal broodstock for pacu breeding programs should be founded with individuals from different fish farms for higher genetic diversity and to avoid inbreeding risks. Expand