Cellulolytic systems in insects.
DNA sequences of cellulase and associated genes, as well as physiological and morphological information about the digestive systems of cellulases-producing insects, may allow the efficient use of cellulosic biomass as a sustainable energy source.
A cellulase gene of termite origin
The first insect cellulase-endoding gene to be identified, RsEG, which encodes an endo-β-1,4-glucanase (EC 188.8.131.52) in the termite Reticulitermes speratus is described.
Evidence from multiple gene sequences indicates that termites evolved from wood-feeding cockroaches
Dual cellulose-digesting system of the wood-feeding termite, Coptotermes formosanus Shiraki.
Major alteration of the expression site of endogenous cellulases in members of an apical termite lineage
- G. Tokuda, N. Lo, Hirofumi Watanabe, Gaku Arakawa, Tadao Matsumoto, H. Noda
- BiologyMolecular Ecology
- 24 August 2004
A comparative study of cellulase expression in various termitid and flagellate‐harbouring species, using enzyme assays and reverse transcription polymerase chain reactions found taxa from phylogenetically basal lineages were consistently found to express endogenous genes specifically in the salivary glands, whilst those from a relatively apical lineage containing termitids expressed cellulases solely in the midgut.
The presence and nature of endogenous cellulases in higher animals are described and the nature of cellulase genes reported from arthropods and nematodes are discussed.
Metazoan cellulase genes from termites: intron/exon structures and sites of expression.
Heterologous expression and enzymatic characterization of β-glucosidase from the drywood-eating termite, Neotermes koshunensis
A β-glucosidase cDNA from the termite, Neotermes koshunensis, was successfully overexpressed in Escherichia coli, and the product was purified to homogeneity by affinity purification against…
Hidden cellulases in termites: revision of an old hypothesis
Zymograms showed that the hindguts of these termites possessed several cellulases and contained up to 59% cellulase activity against crystalline cellulose when compared with the midgut, suggesting that these cellulases were produced by symbiotic bacteria.
Cellulose Digestion in the Wood-Eating Higher Termite, Nasutitermes takasagoensis (Shiraki): Distribution of Cellulases and Properties of Endo-β-1,4-gIucanase
- G. Tokuda, Hirofumi Watanabe, Tadao Matsumoto, H. Noda
- Biology, MedicineZoological Science
- 1 February 1997
The activity and stability on pH and temperature of the purified endo-β-1,4-glucanase are prominent among those from various organisms.