Cellulolytic systems in insects.
- Hirofumi Watanabe, G. Tokuda
- BiologyAnnual Review of Entomology
- 2010
DNA sequences of cellulase and associated genes, as well as physiological and morphological information about the digestive systems of cellulases-producing insects, may allow the efficient use of cellulosic biomass as a sustainable energy source.
A cellulase gene of termite origin
- Hirofumi Watanabe, H. Noda, G. Tokuda, N. Lo
- BiologyNature
- 23 July 1998
The first insect cellulase-endoding gene to be identified, RsEG, which encodes an endo-Ī²-1,4-glucanase (EC 3.2.1.4) in the termite Reticulitermes speratus is described.
Dual cellulose-digesting system of the wood-feeding termite, Coptotermes formosanus Shiraki.
- K. Nakashima, H. Watanabe, H. Saitoh, G. Tokuda, J. Azuma
- BiologyInsect Biochemistry and Molecular Biology
- 1 July 2002
Major alteration of the expression site of endogenous cellulases in members of an apical termite lineage
- G. Tokuda, N. Lo, Hirofumi Watanabe, Gaku Arakawa, Tadao Matsumoto, H. Noda
- BiologyMolecular Ecology
- 24 August 2004
A comparative study of cellulase expression in various termitid and flagellateāharbouring species, using enzyme assays and reverse transcription polymerase chain reactions found taxa from phylogenetically basal lineages were consistently found to express endogenous genes specifically in the salivary glands, whilst those from a relatively apical lineage containing termitids expressed cellulases solely in the midgut.
Animal cellulases
- H. Watanabe, G. Tokuda
- BiologyCellular and Molecular Life Sciences (CMLS)
- 2001
The presence and nature of endogenous cellulases in higher animals are described and the nature of cellulase genes reported from arthropods and nematodes are discussed.
Metazoan cellulase genes from termites: intron/exon structures and sites of expression.
- G. Tokuda, N. Lo, H. Watanabe, M. Slaytor, T. Matsumoto, H. Noda
- BiologyBiochimica et Biophysica Acta
- 28 October 1999
Heterologous expression and enzymatic characterization of Ī²-glucosidase from the drywood-eating termite, Neotermes koshunensis
- J. Ni, G. Tokuda, M. Takehara, Hirofumi Watanabe
- Biology
- 25 August 2007
A Ī²-glucosidase cDNA from the termite, Neotermes koshunensis, was successfully overexpressed in Escherichia coli, and the product was purified to homogeneity by affinity purification againstā¦
Hidden cellulases in termites: revision of an old hypothesis
- G. Tokuda, Hirofumi Watanabe
- BiologyBiology Letters
- 22 June 2007
Zymograms showed that the hindguts of these termites possessed several cellulases and contained up to 59% cellulase activity against crystalline cellulose when compared with the midgut, suggesting that these cellulases were produced by symbiotic bacteria.
Cellulose Digestion in the Wood-Eating Higher Termite, Nasutitermes takasagoensis (Shiraki): Distribution of Cellulases and Properties of Endo-Ī²-1,4-gIucanase
- G. Tokuda, Hirofumi Watanabe, Tadao Matsumoto, H. Noda
- Biology, MedicineZoological Science
- 1 February 1997
The activity and stability on pH and temperature of the purified endo-Ī²-1,4-glucanase are prominent among those from various organisms.
...
...