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Raman, polarized Raman and ultraviolet resonance Raman spectroscopy of nucleic acids and their complexes
Applications of Raman spectroscopy to investigate the molecular constituents of nucleic acids were initiated in the late 1960s and soon thereafter progressed to studies of synthetic and native
Membrane structure and interactions with protein and DNA in bacteriophage PRD1
It is shown that the electron density also reveals the icosahedral lipid bilayer, beneath the protein capsid, enveloping the viral DNA, which is likely to be important during membrane-mediated DNA translocation into the host cell.
Subunit conformations and assembly states of a DNA-translocating motor: the terminase of bacteriophage P22.
A scheme for the roles of P22 terminase large and small subunits in the recruitment and packaging of viral DNA is proposed and the model is discussed in relation to proposals for terminase-driven DNA translocation in other phages.
Raman spectroscopy of DNA-metal complexes. II. The thermal denaturation of DNA in the presence of Sr2+, Ba2+, Mg2+, Ca2+, Mn2+, Co2+, Ni2+, and Cd2+.
A model that describes DNA aggregation and denaturation during heating during heating in the presence of divalent metal cations is led to, which reveals decreases in backbone order, base unstacking, distortion of glycosyl torsion angles, and rupture of hydrogen bonds, which occur after thermal denaturation.
Dependence of the Raman signature of genomic B-DNA on nucleotide base sequence.
The results show that the Raman signature of B DNA depends in detail upon both genomic base composition and sequence, and implies the capability to distinguish genomic DNAs by means of their characteristic Raman signatures.
Raman spectroscopy of DNA-metal complexes. I. Interactions and conformational effects of the divalent cations: Mg, Ca, Sr, Ba, Mn, Co, Ni, Cu, Pd, and Cd.
A relationship between DNA melting and aggregation is proposed in which initial metal binding at major groove sites locally destabilizes the B-DNA double helix, causing displacement of the bases away from one another and exposing additional metal binding sites.
Polarized Raman spectra of oriented fibers of A DNA and B DNA: anisotropic and isotropic local Raman tensors of base and backbone vibrations.
The present fiber results provide a basis for exploitation of polarized Raman spectroscopy to determine DNA helix orientation as well as to probe specific nucleotide residue orientations in nucleoproteins, viruses, and other complex biological assemblies.
Characterization of DNA structures by laser Raman spectroscopy
Oriented fibers drawn from aqueous gels of calf‐thymus DNA were maintained at constant relative humidites of 75 and 92% to yield canonical A‐DNA and B‐DNA structures, respectively. Raman spectra of
The structure of a filamentous bacteriophage.
The first image reconstruction of a filamentous virus, bacteriophage fd, by cryoelectron microscopy is reported, showing that new computational approaches to helical reconstruction can greatly extend the ability to visualize heterogeneous protein polymers at a reasonably high resolution.
Raman markers of nonaromatic side chains in an alpha-helix assembly: Ala, Asp, Glu, Gly, Ile, Leu, Lys, Ser, and Val residues of phage fd subunits.
The findings described here show that nonaromatic side chains contribute prolifically to the fd Raman signature, that marker bands for specific nonaromatics differ in general from those observed in corresponding polypeptides and amino acids, and that the frequencies and intensities of manynonaromatic markers are sensitive to secondary and higher-order structures.