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Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicum.
TLDR
Small mobilizable vectors based on the Escherichia coli plasmids pK18 and pK19 can be transferred by RP4-mediated conjugation into a wide range of Gram- and Gram+ bacteria, and should facilitate gene disruption and allelic exchange by homologous recombination.
The 27.8-kb R-plasmid pTET3 from Corynebacterium glutamicum encodes the aminoglycoside adenyltransferase gene cassette aadA9 and the regulated tetracycline efflux system Tet 33 flanked by active
TLDR
The complete nucleotide sequence of the 27.8-kb R-plasmid pTET3 from Corynebacterium glutamicum LP-6 is determined, suggesting a central role of IS6100 in the evolutionary history of pTet3 by mediating the cointegrative assembly of resistance gene-carrying DNA segments.
Myxothiazol, a new inhibitor of the cytochrome b-c1 segment of th respiratory chain.
TLDR
Comparison spectra with beef heart mitochondria and with cell suspensions of Saccharomyces cerevisiae revealed that myxothiazol blocked the electron transport within the cytochrome b-c1 segment of the respiratory chain.
Influence of increased aspartate availability on lysine formation by a recombinant strain of Corynebacterium glutamicum and utilization of fumarate
TLDR
Undetermined limitations in the sequence of reactions from aspartate to lysine exist in Corynebacterium glutamicum, which indicates that undetermined limitationsIn this strain, fumaric acid was converted to aspartates by fumarate hydratase, malate dehydrogenase, and as partate amino transferase activity.
Plasmids in Corynebacterium glutamicum and their molecular classification by comparative genomics.
TLDR
The deduced mechanism of plasmid DNA replication and the degree of amino acid sequence similarity among replication initiator proteins was the basis for performing a classification of the plasmids into four distinct C. glutamicum plasmID families.
The alanine racemase gene alr is an alternative to antibiotic resistance genes in cloning systems for industrial Corynebacterium glutamicum strains.
TLDR
Experimental evidence is provided that the alanine racemase gene alr can be applied as an alternative selection marker to antibiotic resistance genes in industrial C. glutamicum strains.
The mode of action of stigmatellin, a new inhibitor of the cytochrome b-c1 segment of the respiratory chain☆
TLDR
Experiments with 14 synthesized derivatives of stigmatellin showed that good inhibitory activity can be expected only if the side chain was kept relatively lipophilic, and the keto and the hydroxy groups of the chromone system were left intact.
The myxalamids, new antibiotics from Myxococcus xanthus (Myxobacterales). I. Production, physico-chemical and biological properties, and mechanism of action.
TLDR
From the cell mass and culture supernatant of Myxococcus xanthus strain Mx X12 an antibiotic activity against yeasts, molds and some Gram-positive bacteria could be extracted and the main component was shown to block in beef heart submitochondrial particles the respiratory chain at the site of complex I.
TetZ, a new tetracycline resistance determinant discovered in gram-positive bacteria, shows high homology to gram-negative regulated efflux systems.
TLDR
This is the first time a repressor-regulated tet gene has been found in gram-positive bacteria and a new class of tetracycline resistance and repressor proteins, termed TetA(Z) and TetR(Z), is proposed.
Cloning and nucleotide sequence of the phosphoenolpyruvate carboxylase-coding gene of Corynebacterium glutamicum ATCC13032.
TLDR
At the amino acid level, PEPC from C. glutamicum presents overall a high degree of similarity with corresponding enzymes from three different organisms, and the location of some strictly conserved regions may have important implications for PEPC activity and allostery.
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