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Metabolic Engineering: Principles And Methodologies
The Essence of Metabolic Engineering, a review of Cellular Metabolism, and Examples of Pathway Manipulations: Metabolic engineering in Practice are reviewed.
Reductive glutamine metabolism by IDH1 mediates lipogenesis under hypoxia
It is shown that human cells use reductive metabolism of α-ketoglutarate to synthesize AcCoA for lipid synthesis and support lipid synthesis in mammalian cells.
Isoprenoid Pathway Optimization for Taxol Precursor Overproduction in Escherichia coli
- P. K. Ajikumar, Wenhai Xiao, +7 authors G. Stephanopoulos
- Chemistry, MedicineScience
- 1 September 2010
A multivariate-modular approach to metabolic-pathway engineering that succeeded in increasing titers of taxadiene—the first committed Taxol intermediate—approximately 1 gram per liter in an engineered Escherichia coli strain helped to unlock the potential of the MEP pathway for the engineered production of terpenoid natural products.
Elementary metabolite units (EMU): a novel framework for modeling isotopic distributions.
This work presents a novel framework for the modeling of isotopic labeling systems that significantly reduces the number of system variables without any loss of information and is most efficient for the analysis of labeling by multiple isotopic tracers.
Tuning genetic control through promoter engineering.
- H. Alper, Curt R. Fischer, E. Nevoigt, G. Stephanopoulos
- Biology, MedicineProceedings of the National Academy of Sciences…
- 6 September 2005
The characterized library of promoters is used to assess the impact of phosphoenolpyruvate carboxylase levels on growth yield and deoxy-xylulose-P synthase Levels on lycopene production and is illustrated as being generalizable to eukaryotic organisms and thus constitutes an integral platform for functional genomics, synthetic biology, and metabolic engineering endeavors.
Engineering the push and pull of lipid biosynthesis in oleaginous yeast Yarrowia lipolytica for biofuel production.
This work demonstrates the excellent capacity for lipid production by the oleaginous yeast Y. lipolytica and the effects of metabolic engineering of two important steps of the lipid synthesis pathway, which acts to divert flux towards the cholesterol synthesis and creates driving force for TAG synthesis.
Phosphoglycerate dehydrogenase diverts glycolytic flux and contributes to oncogenesis
It is found that in some cancer cells a relatively large amount of glycolytic carbon is diverted into serine and glycine metabolism through phosphoglycerate dehydrogenase (PHGDH).
Metabolic flux distributions in Corynebacterium glutamicum during growth and lysine overproduction
The two main contributions of this are the solidification of Corynebacterium glutamicum biochemistry guided by bioreaction network analysis, and the determination of bansal metabolic flux…
The mTORC1 Pathway Stimulates Glutamine Metabolism and Cell Proliferation by Repressing SIRT4
It is shown that mTORC1 promotes glutamine anaplerosis by activating glutamate dehydrogenase (GDH) and this data indicate that targeting nutrient metabolism in energy-addicted cancers with high m TORC1 signaling may be an effective therapeutic approach.
Engineering Yeast Transcription Machinery for Improved Ethanol Tolerance and Production
The application of gTME is shown to Saccharomyces cerevisiae for improved glucose/ethanol tolerance, a key trait for many biofuels programs.