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Progressive development of the rat osteoblast phenotype in vitro: Reciprocal relationships in expression of genes associated with osteoblast proliferation and differentiation during formation of the
The relationship of cell proliferation to the temporal expression of genes characterizing a developmental sequence associated with bone cell differentiation was examined in primary diploid cultures
Canonical WNT Signaling Promotes Osteogenesis by Directly Stimulating Runx2 Gene Expression*
It is proposed that WNT/TCF1 signaling, like bone morphogenetic protein/transforming growth factor-β signaling, activates Runx2 gene expression in mesenchymal cells for the control of osteoblast differentiation and skeletal development.
A microRNA signature for a BMP2-induced osteoblast lineage commitment program
The studies establish a mechanism for BMP morphogens to selectively induce a tissue-specific phenotype and suppress alternative lineages by down-regulating multiple miRNAs that constitute an osteogenic program, thereby releasing from inhibition pathway components required for cell lineage commitment.
Molecular mechanisms mediating proliferation/differentiation interrelationships during progressive development of the osteoblast phenotype.
This work states that for many years, bone was defined anatomically and examined largely in a descriptive manner by ultrastructural analysis and by biochemical and histochemical methods, but now, complemented by an increased knowledge of molecular mechanisms that are associated with and regulate expression of genes encoding phenotypic compone...
Relationship of cell growth to the regulation of tissue‐specific gene expression during osteoblast differentiation
The loss of stringent growth control in transformed osteoblasts and in osteosarcoma cells is accompanied by a deregulation of the tightly coupled relationship between proliferation and progressive expression of genes associated with bone cell differentiation.
Self‐renewal of human embryonic stem cells is supported by a shortened G1 cell cycle phase
It is determined that human ES cells remain viable after synchronization with either nocodazole or the anti‐tumor drug Paclitaxel (taxol) and have an abbreviated G1 phase of only 2.5–3 h that is significantly shorter than in somatic cells.
Networks and hubs for the transcriptional control of osteoblastogenesis
An overview of the concepts of tissue-specific transcriptional control mechanisms essential for development of the bone cell phenotype is presented and the specificity of Runx2 regulatory activities provides a basis for novel therapeutic strategies to correct bone disorders.
A program of microRNAs controls osteogenic lineage progression by targeting transcription factor Runx2
It is demonstrated that osteoblastogenesis is limited by an elaborate network of functionally tested miRNAs that directly target the osteogenic master regulator Runx2, and their effects can be reversed by the corresponding anti-miRNAs.
Regulatory controls for osteoblast growth and differentiation: role of Runx/Cbfa/AML factors.
The biological functions of Runx factors in promoting cell fate determination and lineage progression are discussed, which include integrating ECM signaling and cues from developmental, hormonal, and signal transduction pathways by formation of complexes organized in subnuclear domains and mediating cell growth control.
MicroRNA control of bone formation and homeostasis
Characterization of miRNAs that operate through tissue-specific transcription factors in osteoblast and osteoclast lineage cells, as well as intricate feedforward and reverse loops, has provided novel insights into the supervision of signaling pathways and regulatory networks controlling normal bone formation and turnover.