Characterization of human embryonic stem cell lines by the International Stem Cell Initiative
The International Stem Cell Initiative characterized 59 human embryonic stem cell lines from 17 laboratories worldwide. Despite diverse genotypes and different techniques used for derivation and…
Detection of Mycoplasma in cell cultures
It is essential that all new cell cultures entering a laboratory and all cell banks are tested for the presence of Mycoplasma, and two techniques be used, selected from a PCR-based method, indirect staining and an agar and broth culture.
Guidance on Good Cell Culture Practice
The aim of this Guidance on Good Cell Culture Practice is to promote the maintenance of high standards and to reduce uncertainty in the development and application of animal and human cell and tissue culture procedures and products, by encouraging greater international harmonisation, rationalisation and standardisation of laboratory practices.
Guidelines for the use of cell lines in biomedical research
These Guidelines have been prepared to provide those new to the field and others engaged in teaching and instruction with the information necessary to increase their awareness of the problems and to enable them to deal with them effectively.
Good Cell Culture Practice for stem cells and stem-cell-derived models.
This workshop report is considered a first step toward a revised GCCP 2.0 focusing on the increasingly diverse cell types and culture formats used in research, product development, testing and manufacture of biotechnology products and cell-based medicines.
Cryopreservation and Freeze-Drying Protocols
This book discusses Cryopreservation in ex situ conservation of Biological Resources and the role of Biological Resource Centres, as well as the process of Freeze-drying, and some of the techniques used.
Points to consider in the development of seed stocks of pluripotent stem cells for clinical applications: International Stem Cell Banking Initiative (ISCBI).
This guidance is revisited to consider what the requirements would be for delivery of the early seed stocks of stem cell lines intended for clinical applications.
Cryopreservation and banking of mammalian cell lines
This protocol describes the principles and methods used for the preparation of cryopreserved cell stocks to ensure the availability of reproducible cultures for use within a single laboratory at different times and for different collaborating laboratories.
Authentication of animal cell cultures by direct visualization of repetitive DNA, aldolase gene PCR and isoenzyme analysis.
Comparison of defined culture systems for feeder cell free propagation of human embryonic stem cells
- Veronika Akopian, P. Andrews, H. Suemori
- BiologyIn Vitro Cellular & Developmental Biology…
- 26 February 2010
Of the eight cell culture methods assessed, only the control and those based on two commercial media, mTeSR1 and STEMPRO, supported maintenance of most cell lines for ten passages, and the remaining media failed before this point due to lack of attachment, cell death, or overt cell differentiation.