Synthesis of β-alanine from l-aspartate using l-aspartate-α-decarboxylase from Corynebacterium glutamicum
This is the first study concerning the enzymatic production of β-alanine by using ADC, and a pH–stat directed, fed-batch feeding strategy was developed to keep the pH value within 6–7.2 and thus attenuate substrate inhibition.
Genetically switched D-lactate production in Escherichia coli.
Production and characterization of laccase from Pleurotus ferulae in submerged fermentation
The production of laccase was influenced by the initial pH and K+ concentration, and the activity of purified lAccase was enhanced by Cu2+, Mn2+ and Mg2+, and this Pleurotus genus l Accase from P. ferulae JM30X was analyzed by MS spectrum and the results are conducive to furthering the understanding of PleuroTus genusLaccases.
Gene Cloning, Heterologous Expression, and Characterization of a High Maltose-Producing α-Amylase of Rhizopus oryzae
- Song Li, Zhirui Zuo, Zhengxiang Wang
- Biology, MedicineApplied Biochemistry and Biotechnology
- 18 January 2011
The RoAmy hydrolytic activity was the highest on wheat starch but showed only 55% activity on amylopectin relative to soluble corn starch, while the pullulanase activity was negligible, and the main end products of the polysaccharides tested were glucose and maltose.
Role of the Calcium-Binding Residues Asp231, Asp233, and Asp438 in Alpha-Amylase of Bacillus amyloliquefaciens as Revealed by Mutational Analysis
Role of the calcium-binding residues Asp231, Asp233, and Asp438 of Bacillus amyloliquefaciens α-amylase (BAA) on the enzyme properties was investigated by site-directed mutagenesis and resulted in a dramatic reduction in the value of catalytic efficiency (Kcat/Km) and thermo-stability at 60°C.
High-efficient production of citric acid by Aspergillus niger from high concentration of substrate based on the staged-addition glucoamylase strategy
- Baoshi Wang, Hua Li, G. Shi
- Chemistry, MedicineBioprocess and biosystems engineering (Print)
- 7 April 2017
Commercial glucoamylase with high low-pH stability was proposed to staged-add in the fermentation process, which timely compensated enzyme loss, ensuring the glucose supply rate.
Metabolic engineering of Escherichia coli W3110 for L-homoserine production
Heterologous pathway for the production of L-phenylglycine from glucose by E. coli.
Interruption of glycerol pathway in industrial alcoholic yeasts to improve the ethanol production
- Zhong-peng Guo, Liang Zhang, Z. Ding, Zhengxiang Wang, G. Shi
- BiologyApplied Microbiology and Biotechnology
- 1 February 2009
It is possible to improve the ethanol yield by interruption of glycerol pathway in industrial alcoholic yeast by deleting two homologous genes GPD1 and GPD2.