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Complete primary structure of human and rabbit lactase‐phlorizin hydrolase: implications for biosynthesis, membrane anchoring and evolution of the enzyme.
We report the primary structures of human and rabbit brush border membrane beta‐glycosidase complexes (pre‐pro‐lactase‐phlorizin hydrolase, or pre‐pro‐LPH, EC 3.2.1.23‐62), as deduced from cDNAExpand
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A modified procedure for the rapid preparation of efficiently transporting vesicles from small intestinal brush border membranes. Their use in investigating some properties of D-glucose and choline
We have worked out a simplification of the procedure described by Schmitz et al. (Biochim. Biophys. Acta (1973) 323, 98--112) for the preparation of brush border membranes from small intestine. TheExpand
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Measuring elasticity of biological materials by atomic force microscopy
Atomic force microscopy (AFM) has proved its value not only for resolving the topographical structure of biological samples, but also for probing inherent properties of biological structures, likeExpand
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Anchoring and biosynthesis of stalked brush border membrane proteins: glycosidases and peptidases of enterocytes and renal tubuli.
  • G. Semenza
  • Biology, Medicine
  • Annual review of cell biology
  • 1986
INTRODUCTION ... 256 GROSS POSITIONING OF BRUSH BORDER HYDROLASES AND THEIR SOLUBILIZATION 25 9 SUBUNIT STRUCTURE AND MACROMOLECULAR PROPERTIES 263 ANCHORING OF GLYCOSIDASES IN THE MEMBRANE 264 TheExpand
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The sucrase-isomaltase complex: Primary structure, membrane-orientation, and evolution of a stalked, intrinsic brush border protein
The complete primary structure (1827 amino acids) of rabbit intestinal pro-sucrase-isomaltase (pro-SI) was deduced from the sequence of a nearly full-length cDNA. Pro-SI is anchored in the membraneExpand
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Cloning and expression of cDNA for a Na/Pi cotransport system of kidney cortex.
A cDNA library from rabbit kidney cortex was screened for expression of Na-dependent transport of phosphate (Pi) using Xenopus laevis oocytes as an expression system. A single clone was eventuallyExpand
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Phlorizin as a probe of the small-intestinal Na+,D-glucose cotransporter. A model.
(1)'Uptake' of phlorizin by intestinal brush border membrane vesicles is stimulated, much as that of D-glucose, by the simultaneous presence of Naout+ and delta psi much less than 0. However,Expand
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Location of the two catalytic sites in intestinal lactase-phlorizin hydrolase. Comparison with sucrase-isomaltase and with other glycosidases, the membrane anchor of lactase-phlorizin hydrolase.
Lactase-phlorizin hydrolase was isolated by immunoadsorption chromatography from rabbit brush-border membrane vesicles. Inactivation of the enzyme with [3H]conduritol-B-epoxide, a covalent activeExpand
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