Author pages are created from data sourced from our academic publisher partnerships and public sources.
Complete primary structure of human and rabbit lactase‐phlorizin hydrolase: implications for biosynthesis, membrane anchoring and evolution of the enzyme.
We report the primary structures of human and rabbit brush border membrane beta‐glycosidase complexes (pre‐pro‐lactase‐phlorizin hydrolase, or pre‐pro‐LPH, EC 126.96.36.199‐62), as deduced from cDNA… Expand
A modified procedure for the rapid preparation of efficiently transporting vesicles from small intestinal brush border membranes. Their use in investigating some properties of D-glucose and choline…
- M. Kessler, O. Acuto, C. Storelli, H. Murer, M. Mueller, G. Semenza
- Chemistry, Medicine
- Biochimica et biophysica acta
- 4 January 1978
We have worked out a simplification of the procedure described by Schmitz et al. (Biochim. Biophys. Acta (1973) 323, 98--112) for the preparation of brush border membranes from small intestine. The… Expand
Measuring elasticity of biological materials by atomic force microscopy
Atomic force microscopy (AFM) has proved its value not only for resolving the topographical structure of biological samples, but also for probing inherent properties of biological structures, like… Expand
Anchoring and biosynthesis of stalked brush border membrane proteins: glycosidases and peptidases of enterocytes and renal tubuli.
- G. Semenza
- Biology, Medicine
- Annual review of cell biology
INTRODUCTION ... 256 GROSS POSITIONING OF BRUSH BORDER HYDROLASES AND THEIR SOLUBILIZATION 25 9 SUBUNIT STRUCTURE AND MACROMOLECULAR PROPERTIES 263 ANCHORING OF GLYCOSIDASES IN THE MEMBRANE 264 The… Expand
The sucrase-isomaltase complex: Primary structure, membrane-orientation, and evolution of a stalked, intrinsic brush border protein
The complete primary structure (1827 amino acids) of rabbit intestinal pro-sucrase-isomaltase (pro-SI) was deduced from the sequence of a nearly full-length cDNA. Pro-SI is anchored in the membrane… Expand
Biochemistry of the Na+, D-glucose cotransporter of the small-intestinal brush-border membrane. The state of the art in 1984.
Cloning and expression of cDNA for a Na/Pi cotransport system of kidney cortex.
- A. Werner, M. Moore, N. Mantei, J. Biber, G. Semenza, H. Murer
- Biology, Medicine
- Proceedings of the National Academy of Sciences…
- 1 November 1991
A cDNA library from rabbit kidney cortex was screened for expression of Na-dependent transport of phosphate (Pi) using Xenopus laevis oocytes as an expression system. A single clone was eventually… Expand
Phlorizin as a probe of the small-intestinal Na+,D-glucose cotransporter. A model.
(1)'Uptake' of phlorizin by intestinal brush border membrane vesicles is stimulated, much as that of D-glucose, by the simultaneous presence of Naout+ and delta psi much less than 0. However,… Expand
Location of the two catalytic sites in intestinal lactase-phlorizin hydrolase. Comparison with sucrase-isomaltase and with other glycosidases, the membrane anchor of lactase-phlorizin hydrolase.
- H. Wacker, P. Keller, R. Falchetto, G. Legler, G. Semenza
- Medicine, Chemistry
- The Journal of biological chemistry
- 15 September 1992
Lactase-phlorizin hydrolase was isolated by immunoadsorption chromatography from rabbit brush-border membrane vesicles. Inactivation of the enzyme with [3H]conduritol-B-epoxide, a covalent active… Expand
Small intestinal phlorizin hydrolase: the "beta-glycosidase complex".