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Rapid elimination of low-copy DNA sequences in polyploid wheat: a possible mechanism for differentiation of homoeologous chromosomes.
To study genome evolution in allopolyploid plants, we analyzed polyploid wheats and their diploid progenitors for the occurrence of 16 low-copy chromosome- or genome-specific sequences isolated from… Expand
A new opaque variant of maize by a single dominant RNA-interference-inducing transgene.
In maize, alpha-zeins, the main protein components of seed stores, are major determinants of nutritional imbalance when maize is used as the sole food source. Mutations like opaque-2 (o2) are used in… Expand
Purification, Characterization, and Intracellular Localization of Glycosylated Protein Disulfide Isomerase from Wheat Grains
Wheat (Triticum aestivum) storage proteins fold and assemble into complexes that are linked by intra- and intermolecular disulfide bonds, but it is not yet clear whether these processes are… Expand
Nucleotide Sequence of a Wheat cDNA Encoding Protein Disulfide Isomerase
PDI (EC 184.108.40.206) is an ER-resident protein involved in the rearrangement of disulfide bonds of secretory proteins. This protein also appears as one of the two subunits of another ER resident protein,… Expand
Gene tagging with engineered Ds elements in maize.
We describe here protocols for isolating genes in maize using Dissociation (Ds) transposons marked with a green fluorescent protein (GFP) transgene. The introduced marker enables the phenotypic… Expand
Expression of the sorghum 10-member kafirin gene cluster in maize endosperm.
Functional analysis of chromosomal segments containing linked genes requires the insertion of contiguous genomic sequences from bacterial artificial chromosomes (BACs) into the genome. Therefore, we… Expand
Involvement of GroEL in nif gene regulation and nitrogenase assembly.
Several approaches were used to study the role of GroEL, the prototype chaperonin, in the nitrogen fixation (nif) system. An Escherichia coli groEL mutant transformed with the Klebsiella pneumoniae… Expand
Isolation and characterization of chromosome‐specific DNA sequences from a chromosome arm genomic library of common wheat
Summary Isolation, physical mapping and polymorphism of chromosome-specific DNA sequences in wheat are reported. Following the microdissection of the long arm of chromosome 5B (5BL) of common wheat,… Expand
Correlation of genetic and physical structure in the region surrounding the I2Fusarium oxysporum resistance locus in tomato
- G. Segal, M. Sarfatti, M. A. Schaffer, N. Ori, D. Zamir, R. Fluhr
- Biology, Medicine
- Molecular and General Genetics MGG
SummaryThe dominant gene I2 confers on tomato (Lycopersicon esculentum) resistance against the fungus Fusarium oxysporum f. sp. lycopersici race 2. A restriction fragment length polymorphism (RFLP)… Expand
A chromosome-specific sequence common to the B genome of polyploid wheat and Aegilops searsii
A low-copy, non-coding chromosome-specific DNA sequence, isolated from common wheat, was physically mapped to the distal 19% region of the long arm of chromosome 3B (3BL) of common wheat. This… Expand