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Thermodynamics and kinetics of unfolding of the thermostable trimeric adenylate kinase from the archaeon Sulfolobus acidocaldarius.
The thermal stability of adenylate kinase from the thermoacidophilic archaeon Sulfolobus acidocaldarius was characterized comprehensively using denaturant-induced unfolding, differential scanning
The terminal quinol oxidase of the hyperthermophilic archaeon Acidianus ambivalens exhibits a novel subunit structure and gene organization
TLDR
It is concluded that A. ambivalens copes with its strongly acidic environment simply by an extreme turnover of its terminal oxidase, generating a proton gradient only by chemical charge separation.
Chemiosmotic H+ cycling across the plasma membrane of the thermoacidophilic archaebacterium Sulfolobus acidocaldarius
Transitory H+ ejection from Sulfolobus acidocaldarius cells induced by oxygen pulses, and anaerobic H+ backflow were investigated. Aerobic proton extrusion is inhibited by protonophores, by nigericin
Chemiosmotic energy conversion of the archaebacterial thermoacidophile Sulfolobus acidocaldarius: oxidative phosphorylation and the presence of an F0-related N,N'-dicyclohexylcarbodiimide-binding
TLDR
The results reported here promote the view that the archaebacterial ATP-synthesizing complex functionally belongs to the F0F1 class of ATPases, which may be considered as phylogenetically conserved catalysts of energy transduction present in all kingdoms of organisms.
Inhibition of mitochondrial metabolism by the diabetogenic thiadiazine diazoxide. I. Action on succinate dehydrogenase and TCA-cycle oxidations.
Abstract Diazoxide exerts a non-competitive inhibition on succinate dehydrogenase in intact and disintegrated mitochondria. This inhibition causes a diminished rate of citric acid cycle turnover, as
Refined crystal structure of a superoxide dismutase from the hyperthermophilic archaeon Sulfolobus acidocaldarius at 2.2 A resolution.
TLDR
The extremely thermostable superoxide dismutase from the hyperthermophilic archaeon Sulfolobus acidocaldarius was crystallized and the three-dimensional structure was determined by X-ray diffraction methods and secondary structure elements as well as residues important for the catalytic activity of the enzyme were found to be highly conserved.
Archaeal complex II: 'classical' and 'non-classical' succinate:quinone reductases with unusual features.
TLDR
A phylogenetic analysis is presented suggesting a co-evolution of the flavoprotein-binding subunit A and subunit B containing the three iron-sulfur clusters, and a novel class of SQRs is discussed in comparison to the so-called 'classical' complexes.
Glucose transport into spinach chloroplasts.
TLDR
It is concluded that d-glucose and other hexoses are transported by carrier-mediated diffusion across the inner envelope membrane, similar to the transport of d- glucose into erythrocytes.
The structure of a trimeric archaeal adenylate kinase.
TLDR
The adenylate kinase from the hyperthermophilic archaean species Sulfolobus acidocaldarius has been cloned, expressed in Escherichia coli, purified and crystallized and provides a good model for the methanococcal adenYLate kinases.
The structure of the soluble domain of an archaeal Rieske iron-sulfur protein at 1.1 A resolution.
TLDR
The main domain of soxF and part of the cluster-binding domain, though structurally related, show a significantly divergent structure with respect to topology, non-covalent interactions and surface charges, reflecting a different topology of the soxM complex compared to eucaryal bc complexes.
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