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Cloning of usp45, a gene encoding a secreted protein from Lactococcus lactis subsp. lactis MG1363.
- M. van Asseldonk, G. Rutten, M. Oteman, R. Siezen, W. D. de Vos, G. Simons
- Biology, Medicine
- 30 October 1990
We have cloned usp45, a gene encoding an extracellular secretory protein of Lactococcus lactis subsp. lactis strain MG1363. Unidentified secreted 45-kDa protein (Usp45) is secreted by every… Expand
Letters as loot. Confiscated letters filling major gaps in the history of Dutch
Historical sociolinguistics: the field and its future
Abstract This article introduces the new Journal of Historical Sociolinguistics by situating it in the developing field of historical sociolinguistics. The landmark paper of Weinreich et al. (1968),… Expand
Extending the Scope of Construction Grammar
The field of constructionist linguistics is rapidly expanding, as research on a broad variety of language phenomena is increasingly informed by constructionist ideas about grammar. Expand
Unraveling multilingualism in times past
Language, Literature and the Construction of a Dutch National Identity (1780-1830)
The Practice of Letter Writing: Skills, Models, and Early Modern Dutch Manuals
Abstract In this paper, formulaic language in seventeenth- and eighteenth-century Dutch private letters is compared with formulae presented in letter-writing manuals. The most frequent formulae in… Expand
Negation in Seventeenth- and Eighteenth-Century Dutch. A Historical-Sociolinguistic Perspective
The paper discusses the switch from bipartite to single negation in the history of Dutch, where the decisive turning point of this feature is often located in the seventeenth century. Previous… Expand
Overproduction of bovine beta-casein in Escherichia coli and engineering of its main chymosin cleavage site.
- G. Simons, W. van den Heuvel, +6 authors R. Siezen
- Biology, Medicine
- Protein engineering
- 1 September 1993
A cDNA clone containing the entire coding region for bovine beta-casein A3 flanked by 53 base pairs of 5' non-coding and 358 base pairs of 3' non-coding sequences was isolated from a bovine mammary… Expand