Two dimensional thin layer chromatographic separation of polar lipids and determination of phospholipids by phosphorus analysis of spots
Separation of polar lipids by two-dimensional thin layer chromatography providing resolution of all the lipid classes commonly encountered in animal cells and a sensitive, rapid, reproducible…
Quantitative analysis of phospholipids by thin-layer chromatography and phosphorus analysis of spots
The method reported here has the advantage of improved separations by two-dimensionM TLC, direct aspiration of spots by suction, and phosphorus analysis without pr ior elution.
The fatty acid composition of brain sphingolipids: sphingomyelin, ceramide, cerebroside, and cerebroside sulfate.
The fatty acid composition of each sphingolipid was determined by gas-liquid chromatography, and both odd and even chain saturated and monounsaturated fatty acids containing 14 to 26 carbons were present.
Analytical fractionation of complex lipid mixtures: DEAE cellulose column chromatography combined with quantitative thin layer chromatography
Using diethylaminoethyl cellulose column chromatography followed by thin layer chromatography for the fractionation of complex lipid mixtures with results obtained with beef brain and beef heart mitochondrial lipids is described.
Analytical separation of nonlipid water soluble substances and gangliosides from other lipids by dextran gel column chromatography
After passage through the dextran gel columns, lipids eluted from thin-layer chromatograms were found to give infrared spectra identical to those of pure samples obtained by other procedures.
Lipids in the nervous system of different species as a function of age: brain, spinal cord, peripheral nerve, purified whole cell preparations, and subcellular particulates: regulatory mechanisms and…
Quantitative analysis of brain and spinach leaf lipids employing silicic acid column chromatography and acetone for elution of glycolipids
Quantitative elution of acidic and neutral glycolipids of brain and spinach leaves from silicic acid columns with acetone was demonstrated and provided the basis for an analytical procedure employing column and quantitative thin-layer chromatography (TLC).
Lipid composition of beef brain, beef liver, and the sea anemone: Two approaches to quantitative fractionation of complex lipid mixtures
Two new schemes for fractionation of complex lipid mixtures are presented and the types of lipid-nonlipid interactions disclosed by column chromatography and their potential application to biological problems are discussed.
Determination of polar lipids: Quantitative column and thin-layer chromatography
- G. Rouser, G. Kritchevsky, C. Galli, Dorothy Heller
- ChemistryJournal of the American Oil Chemists Society
- 1 March 1965
The structures of the polar lipid classes of plants and animals are presented, their nomenclature discussed, and suggestions are presented for clarification of nomenclature. The three general types…