FM1-43 Dye Behaves as a Permeant Blocker of the Hair-Cell Mechanotransducer Channel
- J. Gale, W. Marcotti, H. Kennedy, C. Kros, G. Richardson
- BiologyJournal of Neuroscience
- 15 September 2001
Extracellular perfusion of FM1-43 reversibly blocks mechanotransduction with half-blocking concentrations in the low micromolar range and the time course for the relief of block after voltage steps to extreme potentials suggests that FM 1-43 competes with other cations for binding sites within the pore of the channel.
Myosin VIIa, harmonin and cadherin 23, three Usher I gene products that cooperate to shape the sensory hair cell bundle
It is proposed that the shaping of the hair bundle relies on a functional unit composed of myosin VIIa, harmonin b and cadherin 23 that is essential to ensure the cohesion of the stereocilia.
The Tip-Link Antigen, a Protein Associated with the Transduction Complex of Sensory Hair Cells, Is Protocadherin-15
The tip-link antigen is identified as an avian ortholog of human protocadherin-15, a hitherto unidentified antigen specifically associated with the tip and kinocilial links of sensory hair bundles in the inner ear and the ciliary calyx of photoreceptors in the eye.
The Very Large G-Protein-Coupled Receptor VLGR1: A Component of the Ankle Link Complex Required for the Normal Development of Auditory Hair Bundles
The results indicate that Vlgr1 is required for formation of the ankle link complex and the normal development of cochlear hair bundles.
Mechano-electrical transducer currents in hair cells of the cultured neonatal mouse cochlea
The first step towards the generation of the receptor potential in hair cells is the gating of the transducer channels and subsequent flow of transducer current, induced by deflection of the…
A quantitative comparison of mechanoelectrical transduction in vestibular and auditory hair cells of neonatal mice
- G. Géléoc, G. Lennan, G. Richardson, C. Kros
- BiologyProceedings of the Royal Society of London…
- 22 April 1997
Relations between the force exerted by the jet, bundle displacement, and the resulting transducer current were studied, and rotation of the hair bundle rather than translation determines the gating of theTransducer channels, independent of bundle height or origin of the cells.
Development and properties of stereociliary link types in hair cells of the mouse cochlea
- R. Goodyear, W. Marcotti, C. Kros, G. Richardson
- BiologyThe Journal of comparative neurology
- 25 April 2005
There is a rich, rapidly changing array of different links covering the developing hair bundle that becomes progressively refined to generate the mature complement by P19, as indicated by the results of electron microscopy.
A Receptor-Like Inositol Lipid Phosphatase Is Required for the Maturation of Developing Cochlear Hair Bundles
Results reveal that Ptprq is required for formation of the shaft connectors of the hair bundle, the normal maturation of cochlear hair bundles, and the long-term survival of high-frequency auditory hair cells.
Easi-CRISPR: a robust method for one-step generation of mice carrying conditional and insertion alleles using long ssDNA donors and CRISPR ribonucleoproteins
Easi-CRISPR solves the major problem of animal genome engineering, namely the inefficiency of targeted DNA cassette insertion, as treating an average of only 50 zygotes is sufficient to produce a correctly targeted allele in up to 100% of live offspring.