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Monoclonal Antibodies against the Adeno-Associated Virus Type 2 (AAV-2) Capsid: Epitope Mapping and Identification of Capsid Domains Involved in AAV-2–Cell Interaction and Neutralization of AAV-2
TLDR
The linear epitopes of A1, A69, and B1 which reside in VP1, VP2, and VP3, respectively, are described using gene fragment phage display library, peptide scan, and peptide competition experiments. Expand
Antibodies to the period gene product of drosophila reveal diverse tissue distribution and rhythmic changes in the visual system
TLDR
The intensity of per-specific staining in the visual system was found to oscillate, defining a free-running circadian rhythm with a peak in the middle of the night. Expand
The period gene of Drosophila carries species‐specific behavioral instructions.
TLDR
The results suggest that the primary amino acid sequence of the per gene encodes species‐specific behavioral instructions that are detectable when only the per Gene is transferred to a different species. Expand
Epitope mapping by phage display: random versus gene-fragment libraries.
TLDR
Fine mapping of the epitopes by peptide scan revealed that the minimal epitopes recognized by mAbBp53-11 and mAbGDO5 consist of four and five amino acids, respectively, whereas mAb215 requires a minimal epitope of 11 amino acids for antigen recognition. Expand
The gene upstream of DmRP128 codes for a novel GTP-binding protein of Drosophila melanogaster
TLDR
It is proposed that 128up is a member of a novel class of GTP-binding proteins, and antibodies against 128up specifically cross-react with mouse 3T3 cells and human Hep2 cells where the subcellular localization of the protein is predominantly perinuclear. Expand
The period clock locus of D. melanogaster codes for a proteoglycan
TLDR
To determine whether the per transcript codes for a proteoglycan, a region of its coding sequence was expressed (in bacteria) as part of a fusion protein, which was used to immunize rabbits and detected an antigen that is present in wild-type flies and absent in a per- mutant. Expand
Structure and Function of the Hetero-oligomeric Cysteine Synthase Complex in Plants*
TLDR
Application of size exclusion chromatography, analytical ultracentrifugation, and isothermal titration calorimetry revealed a hexameric structure of mitochondrial SAT from Arabidopsis thaliana and a 2:1 ratio of the OAS-TL dimer to the SAT hexamer in the CSC. Expand
Retinoblastoma susceptibility gene product pRB activates hypoxia-inducible factor-1 (HIF-1)
TLDR
The domain of pRB identified to bind Hif-1α in vitro is sufficient to cause HIF-1 α transcriptional activation with the further notion that phosphorylation deficient pRB shows stronger HIF -1α transactivation. Expand
Epitope structures recognised by antibodies against the major coat protein (g8p) of filamentous bacteriophage fd (Inoviridae).
TLDR
To map the accessible surface of filamentous bacteriophage fd particles, the epitope structures of polyclonal rabbit serum and three mouse monoclonal antibodies raised against complete phage were analysed and suggest new designs of phage display screening vectors to improve their performance in analysing non-linear epitopes. Expand
Molecular characterization of a putative peroxidase gene of Drosophila melanogaster.
We have identified genomic clones and corresponding cDNAs that encode a putative peroxidase of Drosophila melanogaster. The gene (DmPO) appears as a single copy gene located on the third chromosomeExpand
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