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Pleiotropic mutations in Saccharomyces cerevisiae affecting sterol uptake and metabolism
Sterol uptake control mutants (upc−) have been isolated via ethylmethanesulfonate mutagenesis from wild‐type Saccharomyces cerevisiae. These mutants are heme and sterol competent but possess the… Expand
Two-dimensional electrophoresis and densitometric analysis of solubilized bovine sperm plasma membrane proteins detected by silver staining and radioiodination.
Bovine sperm plasma membranes were extracted with deoxycholate and subjected to two-dimensional gel electrophoresis. Proteins were visualized either by silver staining or autoradiography using 125I.… Expand
Gas chromatographic analysis of intact steryl esters in wild typeSaccharomyces cerevisiae and in an ester accumulating mutant
The steryl ester faction from wild type and mutant strains of the yeastSaccharomyces cerevisiae was analyzed without saponification by a non-polar capillary gas chromatographic column. When expressed… Expand