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The role of modified purine 64 in initiator/elongator discrimination of tRNA(iMet) from yeast and wheat germ.
The role of 2'-ribosylated adenosine 64 in tRNA(iMet) from yeast in initiation/elongation discrimination was investigated. As measured by in vitro translation in rabbit reticulocyte lysate, theExpand
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Stability of triple helices containing RNA and DNA strands: experimental and molecular modeling studies.
UV-absorption spectrophotometry and molecular modeling have been used to study the influence of the chemical nature of sugars (ribose or deoxyribose) on triple helix stability. For theExpand
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The selenocysteine-inserting opal suppressor serine tRNA from E. coli is highly unusual in structure and modification.
Selenocysteine is cotranslationally incorporated into selenoproteins in a unique pathway involving tRNA mediated suppression of a UGA nonsense codon (1-3). The DNA sequence of the gene for thisExpand
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The 3'-terminal end (NCCA) of tRNA determines the structure and stability of the aminoacyl acceptor stem.
We have done a systematic study on the contribution of the single-stranded NCCA end (where N is any nucleotide) to the stability of the aminoacyl stem of tRNA. A 7-bp RNA duplex with theExpand
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Interaction of elongation factor Tu from Escherichia coli with aminoacyl-tRNA carrying a fluorescent reporter group on the 3' terminus.
Transfer ribonucleic acids containing 2-thiocytidine in position 75 ([s2C]tRNAs) were prepared by incorporation of the corresponding cytidine analogue into 3'-shortened tRNA using ATP(CTP):tRNAExpand
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The chemical synthesis of biochemically active oligoribonucleotides using dimethylaminomethylene protected purine H-phosphonates
Abstract Dimethylaminomethylene was applied as the protecting group for the exocyclic amino groups of adenosine and guanosine in the automated chemical synthesis of oligoribonucleotides on a polymerExpand
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Interaction of the isolated domain II/III of Thermus thermophilus elongation factor Tu with the nucleotide exchange factor EF-Ts.
The middle and C-terminal domain (domain II/III) of elongation factor Tu from Thermus thermophilus lacking the GTP/GDP binding domain have been prepared by treating nucleotide-free protein withExpand
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Kirromycin drastically reduces the affinity of Escherichia coli elongation factor Tu for aminoacyl-tRNA.
We have studied the interaction between EF-Tu-GDP or EF-Tu-GTP in complex with kirromycin or aurodox (N1-methylkirromycin) and aminoacyl-tRNA, N-acetylaminoacyl-tRNA, or deacylated tRNA. ThreeExpand
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Interaction of a selenocysteine-incorporating tRNA with elongation factor Tu from E.coli.
Selenocysteine-incorporating tRNA(Sec)(UCA), the product of selC, was isolated from E.coli and aminoacylated with serine. The equilibrium dissociation constant for the interaction ofExpand
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Dimethylaminomethylene protected purine H-phosphonates in the synthesis of biologically active RNA (24-mer)
Preparation of 5’-O-(4,4’-dimethoxytrityl)-2’-O-tert-butyldimethylsilyl (tBDMSi) derivatives of N 2 -dimethylaminomethyleneguanosine ( IIIa ) and N 6 -dimethylaminomethyleneadenosine ( IVa ) andExpand
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