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FACT Facilitates Transcription-Dependent Nucleosome Alteration
The FACT facilitates Pol II–driven transcription by destabilizing nucleosomal structure so that one histone H2A-H2B dimer is removed during enzyme passage, defining the mechanism by which Pol II can transcribe through chromatin without disrupting its epigenetic status. Expand
The general transcription factors of RNA polymerase II.
The chromatin-specific transcription elongation factor FACT comprises human SPT16 and SSRP1 proteins
The biochemical analysis of FACT is provided, providing evidence that Spt16/Cdc68 is involved in transcript elongation in vivo, and FACT specifically interacts with nucleosomes and histone H2A/H2B dimers, indicating that it may work by promoting nucleosome disassembly upon transcription. Expand
A Unified Theory of Gene Expression
Recent progress has revealed that many of the steps in the pathway from gene sequence to active protein are connected, suggesting a unified theory of gene expression. Expand
FACT, a Factor that Facilitates Transcript Elongation through Nucleosomes
The biochemical properties and polypeptide composition of FACT suggest that it is a novel protein factor that facilitates transcript elongation through nucleosome-induced block to productive transcription. Expand
Molecular basis for the recognition of phosphorylated and phosphoacetylated histone h3 by 14-3-3.
The structures of 14-3-3zeta complexed with serine 10-phosphorylated or phosphoacetylated H3 peptides are determined, revealing a distinct mode of 14,3,3/phosphopeptide binding and providing a structural understanding for the lack of effect of acetylation at lysines 9 and 14 on this interaction. Expand
High-resolution mapping of nucleoprotein complexes by site-specific protein-DNA photocrosslinking: organization of the human TBP-TFIIA-TFIIB-DNA quaternary complex.
We have used a novel site-specific protein-DNA photocrosslinking procedure to define the positions of polypeptide chains relative to promoter DNA in binary, ternary, and quaternary complexesExpand
Phenotypic Anchoring of Gene Expression Changes during Estrogen-Induced Uterine Growth
The mechanism by which an estrogen induces organ growth and tissue maturation is defined, and comparison of temporal changes in gene expression and conventional toxicology end points can facilitate the phenotypic anchoring of toxicogenomic data is demonstrated. Expand
Requirement of RSF and FACT for transcription of chromatin templates in vitro.
The minimal factor requirements for activator-dependent transcription on chromatin templates in vitro have been defined and a factor that facilitates Activator- dependent transcription initiation on Chromatin templates was purified. Expand
FACT relieves DSIF/NELF-mediated inhibition of transcriptional elongation and reveals functional differences between P-TEFb and TFIIH.
A novel role is uncovered for FACT in the regulation of transcription on naked DNA that is independent of its activities on chromatin templates and functional differences between P-TEFb and TFIIH in theregulation of transcription are revealed. Expand