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Initiation of biofilm formation in Pseudomonas fluorescens WCS365 proceeds via multiple, convergent signalling pathways: a genetic analysis
Populations of surface‐attached microorganisms comprising either single or multiple species are commonly referred to as biofilms. Using a simple assay for the initiation of biofilm formation (e.g.Expand
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Flagellar and twitching motility are necessary for Pseudomonas aeruginosa biofilm development
The formation of complex bacterial communities known as biofilms begins with the interaction of planktonic cells with a surface in response to appropriate environmental signals. We report theExpand
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Microbial Biofilms: from Ecology to Molecular Genetics
SUMMARY Biofilms are complex communities of microorganisms attached to surfaces or associated with interfaces. Despite the focus of modern microbiology research on pure culture, planktonicExpand
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Mechanisms of biofilm resistance to antimicrobial agents.
Biofilms are communities of microorganisms attached to a surface. It has become clear that biofilm-grown cells express properties distinct from planktonic cells, one of which is an increasedExpand
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Microtiter dish biofilm formation assay.
  • G. O’Toole
  • Biology, Medicine
  • Journal of visualized experiments : JoVE
  • 30 January 2011
Biofilms are communities of microbes attached to surfaces, which can be found in medical, industrial and natural settings. In fact, life in a biofilm probably represents the predominate mode ofExpand
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Genetic approaches to study of biofilms.
Interest in the study of microbial biofilms has increased greatly in recent years due in large part to the profound impact biofilms have in clinical, industrial, and natural settings.Expand
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Rhamnolipids modulate swarming motility patterns of Pseudomonas aeruginosa.
Pseudomonas aeruginosa is capable of twitching, swimming, and swarming motility. The latter form of translocation occurs on semisolid surfaces, requires functional flagella and biosurfactantExpand
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Rhamnolipid surfactant production affects biofilm architecture in Pseudomonas aeruginosa PAO1.
In response to certain environmental signals, bacteria will differentiate from an independent free-living mode of growth and take up an interdependent surface-attached existence. TheseExpand
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Transition from reversible to irreversible attachment during biofilm formation by Pseudomonas fluorescens WCS365 requires an ABC transporter and a large secreted protein
We report the identification of an ATP‐binding cassette (ABC) transporter and an associated large cell‐surface protein that are required for biofilm formation by Pseudomonas fluorescens WCS365. TheExpand
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BifA, a cyclic-Di-GMP phosphodiesterase, inversely regulates biofilm formation and swarming motility by Pseudomonas aeruginosa PA14.
The intracellular signaling molecule, cyclic-di-GMP (c-di-GMP), has been shown to influence bacterial behaviors, including motility and biofilm formation. We report the identification andExpand
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