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Destabilase, the novel epsilon-(gamma-Glu)-Lys isopeptidase with thrombolytic activity.
The salivary gland secretion of the leech Hirudo medicinalis contains the enzyme destabilase which hydrolyses epsilon-(gamma-Glu)-Lys cross-links in stabilized fibrin. Accumulation of Glu residuesExpand
Destabilase complexes ‐ natural liposome produced by medicinal leeches Hirudo medicinalis
Electrophoretic analysis of destabilase preparation demonstrates the presence of protein combinations with MW 12.3, 25 and 50 kD, providing high stability of DC components and a rapid penetration through cellular membranes (transmembrane transfer) and prophylactic antithrombotic action in the case of peroral application to animals. Expand
A stable prostacyclin-like substance produced by the medicinal leech Hirudo medicinalis.
The medicinal leech Hirudo medicinalis produces a low-molecular mass compound with properties similar to those of prostacyclin, which has a molecular mass of 391 Da and can be produced on destruction of the destabilase polypeptide chain. Expand
[Mechanisms of inhibition of vascular-platelet hemostasis by salivary gland secretion of the medicinal leech Hirudo medicinalis].
The mechanism of inhibition of the vascular-platelet stage of hemostasis by medicinal leech salivary gland secretion was studied. It was shown that the secretion blocks platelet adhesion on theExpand
Use of the medicinal leech in the treatment of ear diseases.
Medicinal leeches and the native secretion of their salivary glands diluted with saline were successfully used for the treatment of some ear diseases: tinnitus caused by inner-ear affections, acute external otitis and chronic otitis media. Expand
[Kinetics of L-gamma-Glu-pNA hydrolysis by destabilase, the enzyme from the medicinal leech Hirudo medicinalis].
The molecular mass of destabilase isolated from the medicinae leech Hirudo medicinalis was found to be equal to 12.3 kDa. A kinetic analysis of the sole presently known synthetic substrate,Expand
[Destabilase: an enzyme of medicinal leech salivary gland secretion hydrolyzes the isopeptide bonds in stabilized fibrin].
The salivary gland secretion of the leech Hirudo medicinalis contains an enzyme termed by us as destabilase, which hydrolyzes the epsilon-(gamma-glutamyl)-lysine bonds as a result of fibrinExpand
Salivary gland secretion of the leech Hirudo medicinalis inhibits ADP-induced human platelet adhesion on a collagen-coated surface
The leech Hirudo medieinMis has been used since olden t imes as an agent for removing blood and can be used as a source of inhibitors of proteolytic enzymes thrombin, t rYpsin, and chymot rypsin. Expand
Biological activity and pharmacological properties of anticoagulant complex (Hirudin, plasma kallikrein inhibitor, prostaglandin) fromHirudo Medicinalis
Antiprocoagulant complex isolated from lyophilized medicinal leeches exerted pronounced antithrombotic, thrombolytic, and hypotensive effects in experimental animals after intravenous injection andExpand