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Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA
Analysis of the genomic DNA from a bacterial biofilm grown under aerobic conditions suggests that sulfate-reducing bacteria, despite their anaerobicity, were present in this environment.
The ecology and biotechnology of sulphate-reducing bacteria
Sulphate-reducing bacteria (SRB) are anaerobic microorganisms that use sulphate as a terminal electron acceptor in, for example, the degradation of organic compounds. They are ubiquitous in anoxic…
Application of denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) in microbial ecology
The potentials and limitations of these techniques will be discussed, and it will be indicated why their use in ecological studies has become so important.
Missing lithotroph identified as new planctomycete
The discovery of this missing lithotroph and its identification as a new, autotrophic member of the order Planctomycetales, one of the major distinct divisions of the Bacteria makes an important contribution to the problem of unculturability.
PCR primers to amplify 16S rRNA genes from cyanobacteria
The use of this specific PCR in combination with denaturing gradient gel electrophoresis to probe the diversity of oxygenic phototrophic microorganisms in cultures, lichens, and complex microbial communities is demonstrated.
DGGE/TGGE a method for identifying genes from natural ecosystems.
- G. Muyzer
- Environmental Science, BiologyCurrent opinion in microbiology
- 1 June 1999
Phylogenetic relationships ofThiomicrospira species and their identification in deep-sea hydrothermal vent samples by denaturing gradient gel electrophoresis of 16S rDNA fragments
DGGE analysis of PCR-amplified 16S rDNA fragments was used to explore the genetic diversity of hydrothermal vent microbial communities, specifically to determine the importance of sulfur-oxidizing bacteria therein, and identified three ‘phylotypes’ which represented a newThiomicrospira species, phylogenetically in an intermediate position between Tms.
The anaerobic oxidation of ammonium.
The number of reports on unaccounted high nitrogen losses in wastewater treatment is gradually increasing, indicating that anaerobic ammonium oxidation may be more widespread than previously assumed.
Denaturing gradient gel electrophoresis profiles of 16S rRNA-defined populations inhabiting a hot spring microbial mat community
- M. Ferris, G. Muyzer, D. M. Ward
- Biology, Environmental ScienceApplied and environmental microbiology
- 1 February 1996
DGGE of PCR-amplified 16S rRNA gene segments was used to profile microbial populations inhabiting different temperature regions in the microbial mat community of Octopus Spring, Yellowstone National Park, to suggest that adaptation to temperature has occurred among cyanobacteria which are phylogenetically very similar.