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Molecular genetics of aminoglycoside resistance genes and familial relationships of the aminoglycoside-modifying enzymes.
A preliminary assessment of the amino acids which may be important in binding aminoglycosides was obtained from data and from the results of mutational analysis of several of the genes encoding am inoglycoside-modifying enzymes.
Antibiotic Susceptibility Profiles ofEscherichia coli Strains Lacking Multidrug Efflux Pump Genes
The results identify which MDR pumps contribute to intrinsic resistance under the conditions tested and supply practical information useful for designing sensitive assay strains for cell-based screening of antibacterial compounds.
Detection of Methyltransferases Conferring High-Level Resistance to Aminoglycosides in Enterobacteriaceae from Europe, North America, and Latin America
The alteration of ribosomal targets by recently described 16S rRNA methyltransferases confers resistance to most aminoglycosides, including arbekacin, a rapidly changing event requiring careful monitoring.
The most frequent aminoglycoside resistance mechanisms--changes with time and geographic area: a reflection of aminoglycoside usage patterns? Aminoglycoside Resistance Study Groups.
The aminoglycoside resistance mechanisms revealed by two surveys in Europe and other countries have been compared to those revealed in earlier studies. Mechanisms have become more complex in all
Temporal Appearance of Plasmid-Mediated Quinolone Resistance Genes
One hundred fifty AAC(6′)-Ib-positive gram-negative isolates collected between 1981 and 1991 were examined by PCR for the presence of the aac( 6′)- Ib-cr variant and other plasmid-mediated quinolone resistance (PMQR) genes, making these the earliest known PMQR genes.
Genetic analysis of bacterial acetyltransferases: identification of amino acids determining the specificities of the aminoglycoside 6'-N-acetyltransferase Ib and IIa proteins
A genetic analysis of hybrid AAC(6') proteins indicated that amino acids in the carboxy half of the proteins were largely responsible for determining specificity, and the model that was identified was an aminoglycoside binding domain of these proteins.
Synthesis and Spectrum of the Neoglycoside ACHN-490
ACHN-490 was more active alone in vitro against Pseudomonas aeruginosa and Acinetobacter baumannii isolates with AG-modifying enzymes than against those with altered permeability/efflux and was advanced into clinical development as a new antibacterial agent.
The most frequently occurring aminoglycoside resistance mechanisms--combined results of surveys in eight regions of the world. The Aminoglycoside Resistance Study Groups.
The most common resistance mechanisms in the seven different pathogen groups were summarised and the greatest diversity of resistance mechanisms (67 in 1189 isolates) was found in Acinetobacter, where the most common mechanisms - APH(3')-VI and AAC(3)-? - were generally very rare in other genera but found frequently in Acnetobacter in combinations with a wide variety of other mechanisms.
Combating evolution with intelligent design: the neoglycoside ACHN-490.
Characterization and transcriptional regulation of the 2'-N-acetyltransferase gene from Providencia stuartii
Results suggested that a trans-acting factor, designated aar (for aminoglycoside acetyltransferase regulator), controls AAC(2')-Ia expression in P. stuartii.